Induction of T15-specific suppressor T cells in mice with the CBA/N defect by neonatal injection with anti-T15 idiotype antibody

B. S. Kim, P. B. Massey

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Mice with the CBA/N defect (xid) are unesponsive to phosphorylcholine (PC). To determine whether idiotype-specific suppressor T cells can also be generated in these defective mice, defective (CBA/N x BALB/c)F1 male and nondefective (CBA/N x BALB/c)F1 female or (BALB/c x CBA/N)F1 male mice were neonatally injected with antibodies specific for the major idiotype of anti-PC antibody, i.e., anti-TEPC-15 idiotype (T15id) antibody. Suppressor cell activity was examined by co-culturing spleen cells from neonatally treated F1 mice with spleen cells of normal nondefective F1 mice in the presence of antigen. Spleen cells from defective (CBA/N x BALB/c)F1 mice treated with anti-T15id antibody demonstrated a level of suppressor activity (>83% suppression) comparable to that of similarly treated nondefective F1 mice. This suppression was specific for the T15id of anti-PC response, and a Lyt-1-2+-bearing T cell population appeared to be responsible for the active suppression. These suppressor T cells recognized T15 but not PC, based on a functional absorption test. These results indicate that the CBA/N defects, including the deficiency in the anti-PC response by B lymphocytes and a possible T cell defect, do not influence the generation of T15id-specific suppressor T cells by neonatal injection with anti-T15id antibody.

Original languageEnglish (US)
Pages (from-to)1145-1148
Number of pages4
JournalJournal of Immunology
Volume128
Issue number3
StatePublished - 1982

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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