Infection of Macaca nemestrina by human immunodeficiency virus type-1

Michael B. Agy; Lyn R. Frumkin; Lawrence Corey; Robert W. Coombs; Steven M. Wolinsky; James Koehler; William R. Morton; Michael G. Katze

doi:10.1126/science.1621083

Infection of Macaca nemestrina by human immunodeficiency virus type-1

Michael B. Agy, Lyn R. Frumkin, Lawrence Corey^*, Robert W. Coombs, Steven M. Wolinsky, James Koehler, William R. Morton, Michael G. Katze

^*Corresponding author for this work

Medicine, Infectious Diseases Division

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

After observations that Macaca nemestrina were exceptionally susceptible to simian immunodeficiency virus and human immunodeficiency virus type-2 (HIV-2), studies of HIV-1 replication were initiated. Several strains of HIV-1, including a recent patient isolate, replicated in vitro in peripheral blood mononuclear cells (PBMCs) and in CD4-positive M. nemestrina lymphocytes in a CD4-dependent fashion. Eight animals were subsequently inoculated with either cell-associated or cell-free suspensions of HIV-1. All animals had HIV-1 isolated by cocultivation, had HIV-1 DNA in their PBMCs as shown by polymerase chain reaction, and experienced sustained seroconversion to a broad spectrum of HIV-1 proteins. Macaca nemestrina is an animal model of HIV-1 infections that provides opportunities for evaluating the pathogenesis of acute HIV-1 replication and candidate vaccines and therapies.

Original language	English (US)
Pages (from-to)	103-106
Number of pages	4
Journal	Science
Volume	257
Issue number	5066
DOIs	https://doi.org/10.1126/science.1621083
State	Published - 1992

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title = "Infection of Macaca nemestrina by human immunodeficiency virus type-1",

abstract = "After observations that Macaca nemestrina were exceptionally susceptible to simian immunodeficiency virus and human immunodeficiency virus type-2 (HIV-2), studies of HIV-1 replication were initiated. Several strains of HIV-1, including a recent patient isolate, replicated in vitro in peripheral blood mononuclear cells (PBMCs) and in CD4-positive M. nemestrina lymphocytes in a CD4-dependent fashion. Eight animals were subsequently inoculated with either cell-associated or cell-free suspensions of HIV-1. All animals had HIV-1 isolated by cocultivation, had HIV-1 DNA in their PBMCs as shown by polymerase chain reaction, and experienced sustained seroconversion to a broad spectrum of HIV-1 proteins. Macaca nemestrina is an animal model of HIV-1 infections that provides opportunities for evaluating the pathogenesis of acute HIV-1 replication and candidate vaccines and therapies.",

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AU - Agy, Michael B.

AU - Frumkin, Lyn R.

AU - Corey, Lawrence

AU - Coombs, Robert W.

AU - Wolinsky, Steven M.

AU - Koehler, James

AU - Morton, William R.

AU - Katze, Michael G.

PY - 1992

Y1 - 1992

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AB - After observations that Macaca nemestrina were exceptionally susceptible to simian immunodeficiency virus and human immunodeficiency virus type-2 (HIV-2), studies of HIV-1 replication were initiated. Several strains of HIV-1, including a recent patient isolate, replicated in vitro in peripheral blood mononuclear cells (PBMCs) and in CD4-positive M. nemestrina lymphocytes in a CD4-dependent fashion. Eight animals were subsequently inoculated with either cell-associated or cell-free suspensions of HIV-1. All animals had HIV-1 isolated by cocultivation, had HIV-1 DNA in their PBMCs as shown by polymerase chain reaction, and experienced sustained seroconversion to a broad spectrum of HIV-1 proteins. Macaca nemestrina is an animal model of HIV-1 infections that provides opportunities for evaluating the pathogenesis of acute HIV-1 replication and candidate vaccines and therapies.

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Infection of Macaca nemestrina by human immunodeficiency virus type-1

Abstract

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UN SDGs

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