TY - JOUR
T1 - Infectious response to E. coli
T2 - Molecular and genetic pathways
AU - Schaeffer, Anthony J.
AU - Klumpp, David J.
AU - Weiser, Adam C.
AU - Sengupta, Shomit
AU - Forrestal, Sarah G.
AU - Batler, Robert A.
N1 - Funding Information:
This work was supported by NIDDK award R37 DK42648-09.
PY - 2004/9
Y1 - 2004/9
N2 - Urinary tract infections are most commonly caused by type 1-piliated Escherichia coli (UPEC) and result in urothelial apoptosis, local cytokine release and neutrophil infiltration. A human urothelial cell line was incubated with various E. coli isolates and was then characterized by flow cytometry. UPEC induced rapid urothelial apoptosis that was dependent upon interactions mediated by type 1 pili. Laboratory isolates expressing type 1 pili-induced approximately 50% less apoptosis. UPEC blocked activity of a NF-κB-dependent reporter in response to inflammatory stimuli by stabilizing IκBα and UPEC rapidly altered cellular signalling pathways. Finally, blocking NF-κB activity increased the level of the laboratory strain-induced apoptosis to the level of apoptosis induced by UPEC. These results suggest that UPEC blocks NF-κB and enhances type 1 pili-induced apoptosis as a component of the uropathogenic programme.
AB - Urinary tract infections are most commonly caused by type 1-piliated Escherichia coli (UPEC) and result in urothelial apoptosis, local cytokine release and neutrophil infiltration. A human urothelial cell line was incubated with various E. coli isolates and was then characterized by flow cytometry. UPEC induced rapid urothelial apoptosis that was dependent upon interactions mediated by type 1 pili. Laboratory isolates expressing type 1 pili-induced approximately 50% less apoptosis. UPEC blocked activity of a NF-κB-dependent reporter in response to inflammatory stimuli by stabilizing IκBα and UPEC rapidly altered cellular signalling pathways. Finally, blocking NF-κB activity increased the level of the laboratory strain-induced apoptosis to the level of apoptosis induced by UPEC. These results suggest that UPEC blocks NF-κB and enhances type 1 pili-induced apoptosis as a component of the uropathogenic programme.
KW - Type 1-piliated E. coli
KW - Urinary tract infection
KW - Urothelial apoptosis
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U2 - 10.1016/j.ijantimicag.2004.02.011
DO - 10.1016/j.ijantimicag.2004.02.011
M3 - Article
C2 - 15364309
AN - SCOPUS:4444315942
SN - 0924-8579
VL - 24
SP - 57
EP - 60
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
IS - SUPPL. 1
ER -
