Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis

Marc A Sala, Yalbi Itzel Balderas-Martínez, Ivette Buendía-Roldan, Hiam Abdala-Valencia, Kiwon Nam, Manu Jain, Sangeeta Maruti Bhorade, Ankit Bharat, Paul A. Reyfman, Karen M Ridge, Annie Pardo, Jacob I Sznajder, GR Scott Budinger, Alexander Misharin, Moises Selman

Research output: Contribution to journalArticle

Abstract

Idiopathic pulmonary fibrosis (IPF) is characterized by progressive scarring of the lung parenchyma, leading to respiratory failure and death. High resolution computed tomography of the chest is often diagnostic for IPF, but its cost and the risk of radiation exposure limit its use as a screening tool even in patients at high risk for the disease. In patients with lung cancer, investigators have detected transcriptional signatures of disease in airway and nasal epithelial cells distal to the site of disease that are clinically useful as screening tools. Here we assessed the feasibility of distinguishing patients with IPF from age-matched controls through transcriptomic profiling of nasal epithelial curettage samples, which can be safely and repeatedly sampled over the course of a patient’s illness. We recruited 10 patients with IPF and 23 age-matched healthy control subjects. Using 3′ messenger RNA sequencing (mRNA-seq), we identified 224 differentially expressed genes, most of which were upregulated in patients with IPF compared with controls. Pathway enrichment analysis revealed upregulation of pathways related to immune response and inflammatory signaling in IPF patients compared with controls. These findings support the concept that fibrosis is associated with upregulation of inflammatory pathways across the respiratory epithelium with possible implications for disease detection and pathobiology.

Original languageEnglish (US)
Article number233
JournalRespiratory Research
Volume19
Issue number1
DOIs
StatePublished - Nov 26 2018

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Idiopathic Pulmonary Fibrosis
Nasal Mucosa
Nose
Up-Regulation
RNA Sequence Analysis
Respiratory Mucosa
Curettage
Respiratory Insufficiency
Cicatrix
Lung Neoplasms
Healthy Volunteers
Fibrosis
Thorax
Epithelial Cells
Tomography
Research Personnel
Costs and Cost Analysis
Lung
Messenger RNA
Genes

Keywords

  • Bacteria
  • Immune response
  • Nasal transcriptome
  • Pulmonary fibrosis
  • Virus

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

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title = "Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis",
abstract = "Idiopathic pulmonary fibrosis (IPF) is characterized by progressive scarring of the lung parenchyma, leading to respiratory failure and death. High resolution computed tomography of the chest is often diagnostic for IPF, but its cost and the risk of radiation exposure limit its use as a screening tool even in patients at high risk for the disease. In patients with lung cancer, investigators have detected transcriptional signatures of disease in airway and nasal epithelial cells distal to the site of disease that are clinically useful as screening tools. Here we assessed the feasibility of distinguishing patients with IPF from age-matched controls through transcriptomic profiling of nasal epithelial curettage samples, which can be safely and repeatedly sampled over the course of a patient’s illness. We recruited 10 patients with IPF and 23 age-matched healthy control subjects. Using 3′ messenger RNA sequencing (mRNA-seq), we identified 224 differentially expressed genes, most of which were upregulated in patients with IPF compared with controls. Pathway enrichment analysis revealed upregulation of pathways related to immune response and inflammatory signaling in IPF patients compared with controls. These findings support the concept that fibrosis is associated with upregulation of inflammatory pathways across the respiratory epithelium with possible implications for disease detection and pathobiology.",
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Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis. / Sala, Marc A; Balderas-Martínez, Yalbi Itzel; Buendía-Roldan, Ivette; Abdala-Valencia, Hiam; Nam, Kiwon; Jain, Manu; Bhorade, Sangeeta Maruti; Bharat, Ankit; Reyfman, Paul A.; Ridge, Karen M; Pardo, Annie; Sznajder, Jacob I; Budinger, GR Scott; Misharin, Alexander; Selman, Moises.

In: Respiratory Research, Vol. 19, No. 1, 233, 26.11.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis

AU - Sala, Marc A

AU - Balderas-Martínez, Yalbi Itzel

AU - Buendía-Roldan, Ivette

AU - Abdala-Valencia, Hiam

AU - Nam, Kiwon

AU - Jain, Manu

AU - Bhorade, Sangeeta Maruti

AU - Bharat, Ankit

AU - Reyfman, Paul A.

AU - Ridge, Karen M

AU - Pardo, Annie

AU - Sznajder, Jacob I

AU - Budinger, GR Scott

AU - Misharin, Alexander

AU - Selman, Moises

PY - 2018/11/26

Y1 - 2018/11/26

N2 - Idiopathic pulmonary fibrosis (IPF) is characterized by progressive scarring of the lung parenchyma, leading to respiratory failure and death. High resolution computed tomography of the chest is often diagnostic for IPF, but its cost and the risk of radiation exposure limit its use as a screening tool even in patients at high risk for the disease. In patients with lung cancer, investigators have detected transcriptional signatures of disease in airway and nasal epithelial cells distal to the site of disease that are clinically useful as screening tools. Here we assessed the feasibility of distinguishing patients with IPF from age-matched controls through transcriptomic profiling of nasal epithelial curettage samples, which can be safely and repeatedly sampled over the course of a patient’s illness. We recruited 10 patients with IPF and 23 age-matched healthy control subjects. Using 3′ messenger RNA sequencing (mRNA-seq), we identified 224 differentially expressed genes, most of which were upregulated in patients with IPF compared with controls. Pathway enrichment analysis revealed upregulation of pathways related to immune response and inflammatory signaling in IPF patients compared with controls. These findings support the concept that fibrosis is associated with upregulation of inflammatory pathways across the respiratory epithelium with possible implications for disease detection and pathobiology.

AB - Idiopathic pulmonary fibrosis (IPF) is characterized by progressive scarring of the lung parenchyma, leading to respiratory failure and death. High resolution computed tomography of the chest is often diagnostic for IPF, but its cost and the risk of radiation exposure limit its use as a screening tool even in patients at high risk for the disease. In patients with lung cancer, investigators have detected transcriptional signatures of disease in airway and nasal epithelial cells distal to the site of disease that are clinically useful as screening tools. Here we assessed the feasibility of distinguishing patients with IPF from age-matched controls through transcriptomic profiling of nasal epithelial curettage samples, which can be safely and repeatedly sampled over the course of a patient’s illness. We recruited 10 patients with IPF and 23 age-matched healthy control subjects. Using 3′ messenger RNA sequencing (mRNA-seq), we identified 224 differentially expressed genes, most of which were upregulated in patients with IPF compared with controls. Pathway enrichment analysis revealed upregulation of pathways related to immune response and inflammatory signaling in IPF patients compared with controls. These findings support the concept that fibrosis is associated with upregulation of inflammatory pathways across the respiratory epithelium with possible implications for disease detection and pathobiology.

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KW - Immune response

KW - Nasal transcriptome

KW - Pulmonary fibrosis

KW - Virus

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