Abstract
Idiopathic pulmonary fibrosis (IPF) is characterized by progressive scarring of the lung parenchyma, leading to respiratory failure and death. High resolution computed tomography of the chest is often diagnostic for IPF, but its cost and the risk of radiation exposure limit its use as a screening tool even in patients at high risk for the disease. In patients with lung cancer, investigators have detected transcriptional signatures of disease in airway and nasal epithelial cells distal to the site of disease that are clinically useful as screening tools. Here we assessed the feasibility of distinguishing patients with IPF from age-matched controls through transcriptomic profiling of nasal epithelial curettage samples, which can be safely and repeatedly sampled over the course of a patient’s illness. We recruited 10 patients with IPF and 23 age-matched healthy control subjects. Using 3′ messenger RNA sequencing (mRNA-seq), we identified 224 differentially expressed genes, most of which were upregulated in patients with IPF compared with controls. Pathway enrichment analysis revealed upregulation of pathways related to immune response and inflammatory signaling in IPF patients compared with controls. These findings support the concept that fibrosis is associated with upregulation of inflammatory pathways across the respiratory epithelium with possible implications for disease detection and pathobiology.
Original language | English (US) |
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Article number | 233 |
Journal | Respiratory Research |
Volume | 19 |
Issue number | 1 |
DOIs | |
State | Published - Nov 26 2018 |
Funding
A.V. Misharin is supported by Office of the Assistant Secretary of Defense for Health Affairs, through the Peer Reviewed Medical Research Program under Award W81XWH-15-1-0215, and by NIH NHLBI grants U19 AI135964 and R56 HL135124. A. Bharat is supported by NIH grant HL125940 and matching funds from the Thoracic Surgery Foundation, a research grant from the Society of University Surgeons, and an American Association of Thoracic Surgery John H. Gibbon Jr. Research Scholarship. J.I. Sznajder is supported by NIH grants AG049665, HL048129, HL071643, and HL085534. G.R.S. Budinger is supported by NIH grants ES013995 and HL071643, Veterans Administration grant BX000201, and Office of the Assistant Secretary of Defense for Health Affairs, through the Peer Reviewed Medical Research Program under Award W81XWH-15-1-0215.
Keywords
- Bacteria
- Immune response
- Nasal transcriptome
- Pulmonary fibrosis
- Virus
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
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Additional file 2: of Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis
Sala, M. A. (Creator), Balderas-Martínez, Y. I. (Contributor), Buendía-Roldan, I. (Contributor), Abdala-Valencia, H. (Creator), Nam, K. (Contributor), Jain, M. (Creator), Bhorade, S. (Creator), Bharat, A. (Creator), Reyfman, P. A. (Creator), Ridge, K. M. (Creator), Pardo, A. (Creator), Sznajder, J. I. (Creator), Budinger, G. R. S. (Contributor), Misharin, A. V. (Creator) & Selman, M. (Creator), figshare, 2018
DOI: 10.6084/m9.figshare.7386341, https://springernature.figshare.com/articles/Additional_file_2_of_Inflammatory_pathways_are_upregulated_in_the_nasal_epithelium_in_patients_with_idiopathic_pulmonary_fibrosis/7386341
Dataset
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Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis
Sala, M. A. (Creator), Balderas-Martínez, Y. I. (Contributor), Buendía-Roldan, I. (Creator), Abdala-Valencia, H. (Creator), Nam, K. (Contributor), Jain, M. (Creator), Bhorade, S. (Creator), Bharat, A. (Creator), Reyfman, P. A. (Creator), Ridge, K. M. (Creator), Pardo, A. (Creator), Sznajder, J. I. (Creator), Budinger, G. R. S. (Contributor), Misharin, A. V. (Creator) & Selman, M. (Creator), figshare, 2018
DOI: 10.6084/m9.figshare.c.4313819, https://figshare.com/collections/Inflammatory_pathways_are_upregulated_in_the_nasal_epithelium_in_patients_with_idiopathic_pulmonary_fibrosis/4313819
Dataset
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Additional file 6: of Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis
Sala, M. A. (Creator), Balderas-Martínez, Y. I. (Contributor), Buendía-Roldan, I. (Contributor), Abdala-Valencia, H. (Creator), Nam, K. (Contributor), Jain, M. (Creator), Bhorade, S. (Creator), Bharat, A. (Creator), Reyfman, P. A. (Creator), Ridge, K. M. (Creator), Pardo, A. (Creator), Sznajder, J. I. (Creator), Budinger, G. R. S. (Contributor), Misharin, A. V. (Creator) & Selman, M. (Creator), figshare, 2018
DOI: 10.6084/m9.figshare.7386401, https://springernature.figshare.com/articles/Additional_file_6_of_Inflammatory_pathways_are_upregulated_in_the_nasal_epithelium_in_patients_with_idiopathic_pulmonary_fibrosis/7386401
Dataset