Influence of Cell Morphology on Mesenchymal Stem Cell Transfection

Yingjun Yang; Xinlong Wang; Xiaohong Hu; Naoki Kawazoe; Yingnan Yang; Guoping Chen

doi:10.1021/acsami.8b20490

Influence of Cell Morphology on Mesenchymal Stem Cell Transfection

Yingjun Yang, Xinlong Wang, Xiaohong Hu, Naoki Kawazoe, Yingnan Yang, Guoping Chen^*

^*Corresponding author for this work

MCC Center for Advanced Regenerative Engineering

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Gene transfection has broad applications in bioengineering and biomedical fields. Although many gene carrier materials and transfection methods have been developed, it remains unclear how cell morphology including cell spreading and elongation affects gene transfection. In this study, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on micropatterns and transfected with cationic pAcGFP1-N1 plasmid complexes. The relationship between the cell morphology of hMSCs and gene transfection was investigated using micropatterning techniques. Spreading and elongation of hMSCs were precisely controlled by micropatterned surfaces. The results showed that well-spread and elongated hMSCs had high transfection efficiency. Analysis of the uptake of exogenous genes and DNA synthesis activity indicated that the well-spread and elongated cell morphology promoted gene transfection through enhanced uptake of the cationic complexes and accelerated DNA synthesis. The results should provide useful information for understanding of cell morphology on gene transfection and development of efficient gene transfection methods.

Original language	English (US)
Pages (from-to)	1932-1941
Number of pages	10
Journal	ACS Applied Materials and Interfaces
Volume	11
Issue number	2
DOIs	https://doi.org/10.1021/acsami.8b20490
State	Published - Jan 16 2019

Funding

*E-mail: [email protected]. Tel: +81-29-860-4496. Fax: +81-29-860-4673. ORCID Yingjun Yang: 0000-0003-2746-1192 Yingnan Yang: 0000-0001-8980-0634 Guoping Chen: 0000-0001-6753-3678 Author Contributions Experiment design: G.C., and N.K. Experiments: Y.Y., X.W., and X.H. Data analysis: G.C., N.K., and Y.Y. Manuscript writing: Y.Y., X.W., and G.C. The final version has been approved by all of the authors. Funding This work was supported by the JSPS KAKENHI Grant Numbers 18K19947 and 18K19945. Notes The authors declare no competing financial interest.

Keywords

cell morphology
cellular uptake
mesenchymal stem cell
micropatterning
transfection

ASJC Scopus subject areas

General Materials Science

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10.1021/acsami.8b20490

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title = "Influence of Cell Morphology on Mesenchymal Stem Cell Transfection",

abstract = "Gene transfection has broad applications in bioengineering and biomedical fields. Although many gene carrier materials and transfection methods have been developed, it remains unclear how cell morphology including cell spreading and elongation affects gene transfection. In this study, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on micropatterns and transfected with cationic pAcGFP1-N1 plasmid complexes. The relationship between the cell morphology of hMSCs and gene transfection was investigated using micropatterning techniques. Spreading and elongation of hMSCs were precisely controlled by micropatterned surfaces. The results showed that well-spread and elongated hMSCs had high transfection efficiency. Analysis of the uptake of exogenous genes and DNA synthesis activity indicated that the well-spread and elongated cell morphology promoted gene transfection through enhanced uptake of the cationic complexes and accelerated DNA synthesis. The results should provide useful information for understanding of cell morphology on gene transfection and development of efficient gene transfection methods.",

keywords = "cell morphology, cellular uptake, mesenchymal stem cell, micropatterning, transfection",

author = "Yingjun Yang and Xinlong Wang and Xiaohong Hu and Naoki Kawazoe and Yingnan Yang and Guoping Chen",

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T1 - Influence of Cell Morphology on Mesenchymal Stem Cell Transfection

AU - Yang, Yingjun

AU - Wang, Xinlong

AU - Hu, Xiaohong

AU - Kawazoe, Naoki

AU - Yang, Yingnan

AU - Chen, Guoping

PY - 2019/1/16

Y1 - 2019/1/16

N2 - Gene transfection has broad applications in bioengineering and biomedical fields. Although many gene carrier materials and transfection methods have been developed, it remains unclear how cell morphology including cell spreading and elongation affects gene transfection. In this study, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on micropatterns and transfected with cationic pAcGFP1-N1 plasmid complexes. The relationship between the cell morphology of hMSCs and gene transfection was investigated using micropatterning techniques. Spreading and elongation of hMSCs were precisely controlled by micropatterned surfaces. The results showed that well-spread and elongated hMSCs had high transfection efficiency. Analysis of the uptake of exogenous genes and DNA synthesis activity indicated that the well-spread and elongated cell morphology promoted gene transfection through enhanced uptake of the cationic complexes and accelerated DNA synthesis. The results should provide useful information for understanding of cell morphology on gene transfection and development of efficient gene transfection methods.

AB - Gene transfection has broad applications in bioengineering and biomedical fields. Although many gene carrier materials and transfection methods have been developed, it remains unclear how cell morphology including cell spreading and elongation affects gene transfection. In this study, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on micropatterns and transfected with cationic pAcGFP1-N1 plasmid complexes. The relationship between the cell morphology of hMSCs and gene transfection was investigated using micropatterning techniques. Spreading and elongation of hMSCs were precisely controlled by micropatterned surfaces. The results showed that well-spread and elongated hMSCs had high transfection efficiency. Analysis of the uptake of exogenous genes and DNA synthesis activity indicated that the well-spread and elongated cell morphology promoted gene transfection through enhanced uptake of the cationic complexes and accelerated DNA synthesis. The results should provide useful information for understanding of cell morphology on gene transfection and development of efficient gene transfection methods.

KW - cell morphology

KW - cellular uptake

KW - mesenchymal stem cell

KW - micropatterning

KW - transfection

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Influence of Cell Morphology on Mesenchymal Stem Cell Transfection

Abstract

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Keywords

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