The surface of hydrophobic, polymeric materials frequently nucleate the formation of blood clots due, in part, to the deposition of fibrinogen. To limit this deposition, these surfaces can be coated with densely packed films of certain surfactants. Recently, we discovered that films of surfactants, while limiting the amount of fibrinogen that can bind to a hydrophobic surface, can actually facilitate fibrin formation. It appears that the surfactant molecules influence the interfacial presentation of fibrinogen, making that protein more reactive/coagulable than it would otherwise be in the absence of the amphiphile. To assess the influence of bound surfactants on the adsorption and coagulability of fibrinogen, microscopic poly(styrene-divinylbenzene) beads were coated from aqueous solutions using a series of poly(ethylene oxide)/poly(propylene oxide) copolymers. The copolymer-coated beads were then exposed to 125I-fibrinogen, and the total amount of fibrinogen adsorbed to the surface was quantitated. After contact with fibrinogen, the beads were placed in an aggregometer where, in the presence of thrombin, a bead aggregate occurred if fibrin dimers formed between beads. While all of the surfactant-modified beads bound fibrinogen, the aggregability of the particles was different. Available evidence indicates that these differences are due to differences in the orientation/conformation of the fibrinogen bound to the surfactant-modified surfaces.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology