Influence of Electrostatics on Small Molecule Flux through a Protein Nanoreactor

Jeff E. Glasgow, Michael A. Asensio, Christopher M. Jakobson, Matthew B. Francis, Danielle Tullman-Ercek*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Nature uses protein compartmentalization to great effect for control over enzymatic pathways, and the strategy has great promise for synthetic biology. In particular, encapsulation in nanometer-sized containers to create nanoreactors has the potential to elicit interesting, unexplored effects resulting from deviations from well-understood bulk processes. Self-assembled protein shells for encapsulation are especially desirable for their uniform structures and ease of perturbation through genetic mutation. Here, we use the MS2 capsid, a well-defined porous 27 nm protein shell, as an enzymatic nanoreactor to explore pore-structure effects on substrate and product flux during the catalyzed reaction. Our results suggest that the shell can influence the enzymatic reaction based on charge repulsion between small molecules and point mutations around the pore structure. These findings also lend support to the hypothesis that protein compartments modulate the transport of small molecules and thus influence metabolic reactions and catalysis in vitro.

Original languageEnglish (US)
Pages (from-to)1011-1019
Number of pages9
JournalACS synthetic biology
Volume4
Issue number9
DOIs
StatePublished - Sep 18 2015

Keywords

  • compartmentalization
  • enzyme catalysis
  • enzyme encapsulation
  • nanobioreactor
  • virus

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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