Influence of serum starvation of rabbit fetal fibroblasts on nuclear reprogramming efficiency

V. V. Galat*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Successful transplantation of mammalian nuclei from differentiated cells has become possible after the application of original methods directed at the synchronization of cell cycles of the donor cell and recipient cytoplasm. We obtained a line of rabbit fetal fibroblasts which was used to study factors affecting the success of reprogramming. The nuclei of fetal fibroblasts (up to the 10th passage inclusive) proved to be capable of reprogramming and ensuring development of the cloned embryos until the preimplantation stages. The influence of synchronization of the cell cycles of the nucleus donor and recipient on the efficiency of reprogramming was studied. The rate of development of the cloned rabbit embryos to the morula-blastocyst stage reached 67% when the nuclei used were from stationary culture cells (G0-phase).

Original languageEnglish (US)
Pages (from-to)411-416
Number of pages6
JournalOntogenez
Volume30
Issue number6
StatePublished - Dec 1 1999

Keywords

  • Fetal fibroblasts
  • Nuclear transplantation
  • Oocytes
  • Rabbit
  • Reconstructed embryos
  • Reprogramming of nuclei

ASJC Scopus subject areas

  • Medicine(all)

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