Influenza virus hemagglutinin and neuraminidase cytoplasmic tails control particle shape

Hong Jin, George P. Leser, Jie Zhang, Robert A. Lamb*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

206 Scopus citations

Abstract

The cytoplasmic tails of the influenza virus glycoproteins hemagglutinin (HA) and neuraminidase (NA) are highly conserved in sequence for all virus subtypes and it is believed that assembly of this enveloped virus depends on interactions of these domains with cytoplasmic viral components. However, it is possible to rescue altered influenza viruses lacking either the HA or NA cytoplasmic tails. We have obtained an influenza virus that lacks both the cytoplasmic tail of HA and NA. Particle production is reduced ~ 10-fold but these particles, although having a fairly normal protein composition, are greatly elongated and of extended irregular shape. We propose a model in which the interactions of the cytoplasmic tails of HA and NA with an internal viral component are so important for spherical virion shape that there is dual redundancy in the interactions.

Original languageEnglish (US)
Pages (from-to)1236-1247
Number of pages12
JournalEMBO Journal
Volume16
Issue number6
DOIs
StatePublished - Mar 17 1997

Keywords

  • Cytoplasmic tails
  • Hemagglutinin
  • Influenza virus
  • Neuraminidase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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