Abstract
Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in infectious titers, at 24. h or 48. h post-infection. In contrast, tetherin expression inhibited influenza virus-like particle (VLP) release into the media. Expression of the HIV protein Vpu overcame the tetherin block of influenza virus VLPs. A human tetherin mutant that lacks a C-terminal GPI anchor attachment signal (tetherin-ΔGPI) was constructed to test if this mutant could be incorporated into the released virus or VLP particles. Whereas tetherin-ΔGPI was incorporated into influenza VLPs it was not incorporated into influenza virions. Taken together these data suggest that influenza virions may contain a tetherin antagonist.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 50-56 |
| Number of pages | 7 |
| Journal | Virology |
| Volume | 417 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 15 2011 |
Funding
We thank Dr. Paul Bieniasz for the tetherin plasmid, Dr. Klaus Strebel for Vpu plasmid and Dr. John K. Rose for VSV/∆G/GFP. The electron microscopy was performed in the Northwestern University Biological Imaging Facility (Evanston Campus). R.W. was an HHMI associate, G.P.L is an HHMI Research Specialist and R.A.L. is an HHMI Investigator. This research was supported in part by Research Grant AI-20201-29 from the National Institute of Allergy and Infectious Diseases .
Keywords
- BST2
- CD317
- Influenza virus
- Restriction factors
- Tetherin
- Virus restriction
- Virus-like particles
ASJC Scopus subject areas
- Virology
