Influenza virus M2 ion channel protein is necessary for filamentous virion formation

Jeremy S. Rossman, Xianghong Jing, George P. Leser, Victoria Balannik, Lawrence H. Pinto, Robert A. Lamb

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Influenza A virus buds from cells as spherical (∼100-nm diameter) and filamentous (∼100 nm X 2 to 20 μm) virions. Previous work has determined that the matrix protein (M1) confers the ability of the virus to form filaments; however, additional work has suggested that the influenza virus M2 integral membrane protein also plays a role in viral filament formation. In examining the role of the M2 protein in filament formation, we observed that the cytoplasmic tail of M2 contains several sites that are essential for filament formation. Additionally, whereas M2 is a nonraft protein, expression of other viral proteins in the context of influenza virus infection leads to the colocalization of M2 with sites of virus budding and lipid raft domains. We found that an amphipathic helix located within the M2 cytoplasmic tail is able to bind cholesterol, and we speculate that M2 cholesterol binding is essential for both filament formation and the stability of existing viral filaments.

Original languageEnglish (US)
Pages (from-to)5078-5088
Number of pages11
JournalJournal of virology
Volume84
Issue number10
DOIs
StatePublished - May 1 2010

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Influenza virus M2 ion channel protein is necessary for filamentous virion formation'. Together they form a unique fingerprint.

  • Cite this