Infrared imaging of nitric oxide-mediated blood flow in human sickle cell disease

Alexander M. Gorbach, Hans C. Ackerman*, Wei Min Liu, Joseph M. Meyer, Patricia L. Littel, Catherine Seamon, Eleni Footman, Amy Chi, Suzana Zorca, Megan L. Krajewski, Michael J. Cuttica, Roberto F. Machado, Richard O. Cannon, Gregory J. Kato

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Vascular dysfunction is an important pathophysiologic manifestation of sickle cell disease (SCD), a condition that increases risk of pulmonary hypertension and stroke. We hypothesized that infrared (IR) imaging would detect changes in cutaneous bloodflow reflective of vascular function. We performed IR imaging and conventional strain gauge plethysmography in twenty-five adults with SCD at baseline and during intra-arterial infusions of an endothelium-dependent vasodilator acetylcholine (ACh), an endothelium-independent vasodilator sodium nitroprusside (SNP), and a NOS inhibitor L-NMMA. Skin temperature measured by IR imaging increased in a dose-dependent manner to graded infusions of ACh (+1.1°C, p<0.0001) and SNP (+0.9°C, p<0.0001), and correlated with dose-dependent increases in forearm blood flow (ACh: +19.9mL/min/100mL, p<0.0001; rs=0.57, p=0.003; SNP: +8.6mL/min/100mL, p<0.0001; r=0.70, p=0.0002). Although IR measurement of skin temperature accurately reflected agonist-induced increases in blood flow, it was less sensitive to decreases in blood flow caused by NOS inhibition. Baseline forearm skin temperature measured by IR imaging correlated significantly with baseline forearm blood flow (31.8±0.2°C, 6.0±0.4mL/min/100mL; r=0.58, p=0.003), and appeared to represent a novel biomarker of vascular function. It predicted a blunted blood flow response to SNP (r=-0.61, p=0.002), and was independently associated with a marker of pulmonary artery pressure, as well as hemoglobin level, diastolic blood pressure, homocysteine, and cholesterol (R2=0.84, p<0.0001 for the model). IR imaging of agonist-stimulated cutaneous blood flow represents a less cumbersome alternative to plethysmography methodology. Measurement of baseline skin temperature by IR imaging may be a useful new marker of vascular risk in adults with SCD.

Original languageEnglish (US)
Pages (from-to)262-269
Number of pages8
JournalMicrovascular Research
Issue number3
StatePublished - Nov 2012

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Biochemistry
  • Cell Biology


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