Infrequent p53 gene mutations and lack of p53 protein expression in clear cell sarcoma of the kidney: Immunohistochemical study and mutation analysis of p53 in renal tumors of unfavorable prognosis

Chuen Hsueh, Hao Wang, Frank Gonzalez-Crussi, Jer Nan Lin, Iou Jih Hung, Chao Ping Yang, Tang Her Jiang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A high prevalence of p53 gene mutation and protein expression has been found in the anaplastic variant of Wilms' tumor (WT), known to be associated with poor outcome. However, there are very few studies of p53 alterations in the other two rare and highly malignant renal tumors in childhood, in other words, clear cell sarcoma of the kidney (CCSK) and malignant rhabdoid tumor of the kidney (MRTK). Overexpression of p53 protein has been detected in eight CCSKs in one study, and in two in another, yet no molecular correlation with p53 gene mutations has been carried out. Our study is the first molecular analysis concerning p53 in CCSK. We investigated eight cases of CCSK and one case of MRTK for p53 protein expression by immunohistochemical staining. All were analyzed for p53 mutations in the region of exons 4 to 8 by polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) method and DNA sequencing analysis. By histological study, no CCSK showed anaplastic features. None expressed p53 protein, but two harbored p53 mutations. One was in exon 5, with a base pair insertion between codons 162 to 163 causing frameshift alteration in amino acid. Another was a silent CTC→CTT transversion in codon 289 of exon 8. The case of MRTK did not show any alterations of p53 protein or gene. Our result indicates that p53 alterations are infrequent in CCSK and do not seem to be primary genetic events in the pathogenesis of CCSK.

Original languageEnglish (US)
Pages (from-to)606-610
Number of pages5
JournalModern Pathology
Volume15
Issue number6
DOIs
StatePublished - 2002

Keywords

  • Anaplastic
  • Clear cell sarcoma of the kidney
  • Malignant rhabdoid tumor of the kidney
  • P53
  • Wilms' tumor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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