Inhaled nitric oxide increases endothelin-1 levels: A potential cause of rebound pulmonary hypertension

Jeffrey M. Pearl*, David P. Nelson, Jenni L. Raake, Peter B. Manning, Steven M. Schwartz, Lisa Koons, Thomas P. Shanley, Hector R. Wong, Jodie Y. Duffy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Objective: Inhaled nitric oxide (iNO) is front-line therapy for pulmonary hypertension after repair of congenital heart disease. However, little clinical data exists regarding the effects of iNO on regulators of pulmonary vascular resistance. An imbalance between primary vasodilators, such as NO, and vasoconstrictors, such as endothelin-1 (ET-1), has been implicated in rebound pulmonary hypertension upon iNO withdrawal. The objective of this study was to determine whether iNO therapy alters plasma ET-1 levels. Design: This is a prospective study involving pediatric and adult patients at risk for pulmonary hypertension. Setting: Pediatric patients were in the cardlac intensive care unit and adult patients were in a tertiary-care hospital. Patients: Group 1 included children with congenital heart disease requiring iNO for treatment of pulmonary hypertension after cardiopulmonary bypass (n = 15), group 2 was adults receiving iNO (n = 10), and group 3 included children at risk for pulmonary hypertension after bypass that did not require iNO (n = 8). Interventions: Dosages of iNO were 2-60 ppm. The duration of therapy ranged from 23 to 188 hrs in group 1 and 29 to 108 hrs in group 2. Measurements and Main Results: Arterial blood was obtained for the measurement of ET-1 levels before and during iNO therapy and 24 hrs after iNO withdrawal, Group 1 mean ET-1 levels increased to 127% of baseline by 12 hrs of iNO, remained elevated at 48 hrs (p < .05), then decreased to 71% of iNO levels 24 hrs after withdrawal (p < .01). Group 2 ET-1 levels increased to 147%, and 137% of baseline at 12 and 24 hrs of iNO therapy, then fell to 68% of baseline within 24 hrs of discontinuing iNO. ET-1 levels in group 3 decreased after surgery (p < .05). Conclusions: These data suggest that iNO increased plasma ET-1 levels, which subsequently decreased when iNO was discontinued. Increased circulating ET-1 levels might contribute to rebound pulmonary hypertension upon iNO withdrawal.

Original languageEnglish (US)
Pages (from-to)89-93
Number of pages5
JournalCritical care medicine
Issue number1
StatePublished - Jan 1 2002


  • Acute lung injury
  • Endothelin-1
  • Inhaled nitric oxide
  • Nitric oxide
  • Pulmonary hypertension
  • Pulmonary vascular resistance

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine


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