Inherent steroid 17α,20-lyase activity in defunct cytochrome P450 17A enzymes

Eric Gonzalez, Kevin M. Johnson, Pradeep S. Pallan, Thanh T.N. Phan, Wei Zhang, Li Lei, Zdzislaw Wawrzak, Francis K. Yoshimoto, Martin Egli, F. Peter Guengerich*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cytochrome P450 (P450) 17A1 catalyzes the oxidations of progesterone and pregnenolone and is the major source of androgens. The enzyme catalyzes both 17α-hydroxylation and a subsequent 17α,20-lyase reaction, and several mechanisms have been proposed for the latter step. Zebrafish P450 17A2 catalyzes only the 17α-hydroxylations. We previously reported high similarity of the crystal structures of zebrafish P450 17A1 and 17A2 and human P450 17A1. Five residues near the heme, which differed, were changed. We also crystallized this five-residue zebrafish P450 17A1 mutant, and the active site still resembled the structure in the other proteins, with some important differences. These P450 17A1 and 17A2 mutants had catalytic profiles more similar to each other than did the wildtype proteins. Docking with these structures can explain several minor products, which require multiple enzyme conformations. The 17α-hydroperoxy (OOH) derivatives of the steroids were used as oxygen surrogates. Human P450 17A1 and zebrafish P450s 17A1 and P450 17A2 readily converted these to the lyase products in the absence of other proteins or cofactors (with catalytically competent kinetics) plus hydroxylated 17α-hydroxysteroids. The 17α-OOH results indicate that a "Compound I" (FeO3+) intermediate is capable of formation and can be used to rationalize the products. We conclude that zebrafish P450 17A2 is capable of lyase activity with the 17α-OOH steroids because it can achieve an appropriate conformation for lyase catalysis in this system that is precluded in the conventional reaction.

Original languageEnglish (US)
Pages (from-to)541-556
Number of pages16
JournalJournal of Biological Chemistry
Volume293
Issue number2
DOIs
StatePublished - Jan 12 2018

Fingerprint

Steroid 17-alpha-Hydroxylase
Lyases
Zebrafish
Cytochrome P-450 Enzyme System
Hydroxylation
Steroids
Conformations
Hydroxysteroids
Pregnenolone
Proteins
Enzymes
Heme
Catalysis
Androgens
Progesterone
Crystal structure
Oxygen
Derivatives
Oxidation
Kinetics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Gonzalez, E., Johnson, K. M., Pallan, P. S., Phan, T. T. N., Zhang, W., Lei, L., ... Peter Guengerich, F. (2018). Inherent steroid 17α,20-lyase activity in defunct cytochrome P450 17A enzymes. Journal of Biological Chemistry, 293(2), 541-556. https://doi.org/10.1074/jbc.RA117.000504
Gonzalez, Eric ; Johnson, Kevin M. ; Pallan, Pradeep S. ; Phan, Thanh T.N. ; Zhang, Wei ; Lei, Li ; Wawrzak, Zdzislaw ; Yoshimoto, Francis K. ; Egli, Martin ; Peter Guengerich, F. / Inherent steroid 17α,20-lyase activity in defunct cytochrome P450 17A enzymes. In: Journal of Biological Chemistry. 2018 ; Vol. 293, No. 2. pp. 541-556.
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abstract = "Cytochrome P450 (P450) 17A1 catalyzes the oxidations of progesterone and pregnenolone and is the major source of androgens. The enzyme catalyzes both 17α-hydroxylation and a subsequent 17α,20-lyase reaction, and several mechanisms have been proposed for the latter step. Zebrafish P450 17A2 catalyzes only the 17α-hydroxylations. We previously reported high similarity of the crystal structures of zebrafish P450 17A1 and 17A2 and human P450 17A1. Five residues near the heme, which differed, were changed. We also crystallized this five-residue zebrafish P450 17A1 mutant, and the active site still resembled the structure in the other proteins, with some important differences. These P450 17A1 and 17A2 mutants had catalytic profiles more similar to each other than did the wildtype proteins. Docking with these structures can explain several minor products, which require multiple enzyme conformations. The 17α-hydroperoxy (OOH) derivatives of the steroids were used as oxygen surrogates. Human P450 17A1 and zebrafish P450s 17A1 and P450 17A2 readily converted these to the lyase products in the absence of other proteins or cofactors (with catalytically competent kinetics) plus hydroxylated 17α-hydroxysteroids. The 17α-OOH results indicate that a {"}Compound I{"} (FeO3+) intermediate is capable of formation and can be used to rationalize the products. We conclude that zebrafish P450 17A2 is capable of lyase activity with the 17α-OOH steroids because it can achieve an appropriate conformation for lyase catalysis in this system that is precluded in the conventional reaction.",
author = "Eric Gonzalez and Johnson, {Kevin M.} and Pallan, {Pradeep S.} and Phan, {Thanh T.N.} and Wei Zhang and Li Lei and Zdzislaw Wawrzak and Yoshimoto, {Francis K.} and Martin Egli and {Peter Guengerich}, F.",
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Gonzalez, E, Johnson, KM, Pallan, PS, Phan, TTN, Zhang, W, Lei, L, Wawrzak, Z, Yoshimoto, FK, Egli, M & Peter Guengerich, F 2018, 'Inherent steroid 17α,20-lyase activity in defunct cytochrome P450 17A enzymes', Journal of Biological Chemistry, vol. 293, no. 2, pp. 541-556. https://doi.org/10.1074/jbc.RA117.000504

Inherent steroid 17α,20-lyase activity in defunct cytochrome P450 17A enzymes. / Gonzalez, Eric; Johnson, Kevin M.; Pallan, Pradeep S.; Phan, Thanh T.N.; Zhang, Wei; Lei, Li; Wawrzak, Zdzislaw; Yoshimoto, Francis K.; Egli, Martin; Peter Guengerich, F.

In: Journal of Biological Chemistry, Vol. 293, No. 2, 12.01.2018, p. 541-556.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inherent steroid 17α,20-lyase activity in defunct cytochrome P450 17A enzymes

AU - Gonzalez, Eric

AU - Johnson, Kevin M.

AU - Pallan, Pradeep S.

AU - Phan, Thanh T.N.

AU - Zhang, Wei

AU - Lei, Li

AU - Wawrzak, Zdzislaw

AU - Yoshimoto, Francis K.

AU - Egli, Martin

AU - Peter Guengerich, F.

PY - 2018/1/12

Y1 - 2018/1/12

N2 - Cytochrome P450 (P450) 17A1 catalyzes the oxidations of progesterone and pregnenolone and is the major source of androgens. The enzyme catalyzes both 17α-hydroxylation and a subsequent 17α,20-lyase reaction, and several mechanisms have been proposed for the latter step. Zebrafish P450 17A2 catalyzes only the 17α-hydroxylations. We previously reported high similarity of the crystal structures of zebrafish P450 17A1 and 17A2 and human P450 17A1. Five residues near the heme, which differed, were changed. We also crystallized this five-residue zebrafish P450 17A1 mutant, and the active site still resembled the structure in the other proteins, with some important differences. These P450 17A1 and 17A2 mutants had catalytic profiles more similar to each other than did the wildtype proteins. Docking with these structures can explain several minor products, which require multiple enzyme conformations. The 17α-hydroperoxy (OOH) derivatives of the steroids were used as oxygen surrogates. Human P450 17A1 and zebrafish P450s 17A1 and P450 17A2 readily converted these to the lyase products in the absence of other proteins or cofactors (with catalytically competent kinetics) plus hydroxylated 17α-hydroxysteroids. The 17α-OOH results indicate that a "Compound I" (FeO3+) intermediate is capable of formation and can be used to rationalize the products. We conclude that zebrafish P450 17A2 is capable of lyase activity with the 17α-OOH steroids because it can achieve an appropriate conformation for lyase catalysis in this system that is precluded in the conventional reaction.

AB - Cytochrome P450 (P450) 17A1 catalyzes the oxidations of progesterone and pregnenolone and is the major source of androgens. The enzyme catalyzes both 17α-hydroxylation and a subsequent 17α,20-lyase reaction, and several mechanisms have been proposed for the latter step. Zebrafish P450 17A2 catalyzes only the 17α-hydroxylations. We previously reported high similarity of the crystal structures of zebrafish P450 17A1 and 17A2 and human P450 17A1. Five residues near the heme, which differed, were changed. We also crystallized this five-residue zebrafish P450 17A1 mutant, and the active site still resembled the structure in the other proteins, with some important differences. These P450 17A1 and 17A2 mutants had catalytic profiles more similar to each other than did the wildtype proteins. Docking with these structures can explain several minor products, which require multiple enzyme conformations. The 17α-hydroperoxy (OOH) derivatives of the steroids were used as oxygen surrogates. Human P450 17A1 and zebrafish P450s 17A1 and P450 17A2 readily converted these to the lyase products in the absence of other proteins or cofactors (with catalytically competent kinetics) plus hydroxylated 17α-hydroxysteroids. The 17α-OOH results indicate that a "Compound I" (FeO3+) intermediate is capable of formation and can be used to rationalize the products. We conclude that zebrafish P450 17A2 is capable of lyase activity with the 17α-OOH steroids because it can achieve an appropriate conformation for lyase catalysis in this system that is precluded in the conventional reaction.

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