Inhibition of amyloid-β-induced neurotoxicity and apoptosis by moderate ethanol preconditioning

Abdelhak Belmadani, Sripathirathan Kumar, Matthew Schipma, Michael A. Collins, Edward J. Neafsey*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Consumers of moderate amounts of ethanol have a lower risk of Alzheimer's dementia than do abstainers. In Alzheimer's disease the brain contains many extracellular plaques composed of amyloid-β (Aβ), a neurotoxic protein linked to pathogenesis of the disease. Here we report that moderate ethanol preconditioning (20-30 mM for 6 days) of organotypic hippocampal-entorhinal slice cultures prevents Aβ-induced neurotoxicity and apoptosis as measured by media lactate dehydrogenase levels and staining with propidium iodide and Hoechst 33342. With Aβ, as with our previous studies of the neurotoxic HIV-1 protein gp120, moderate ethanol preconditioning may interfere with various glial-mediated neurotoxic responses in the slices to Aβ. In addition, we found that moderate ethanol preconditioning causes an almost 3-fold increase in brain levels of heat shock protein 70 (hsp70), a protective molecular chaperone. Our results suggest possible molecular mechanisms underlying the protective effect of moderate drinking against Alzheimer's dementia.

Original languageEnglish (US)
Pages (from-to)2093-2096
Number of pages4
JournalNeuroreport
Volume15
Issue number13
DOIs
StatePublished - Sep 2004

Keywords

  • Amyloid-beta
  • Ethanol
  • Preconditioning

ASJC Scopus subject areas

  • Neuroscience(all)

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