Inhibition of calcium oxalate crystal growth in vitro by uropontin: Another member of the aspartic acid-rich protein superfamily

H. Shiraga, W. Min, W. J. Vandusen, M. D. Clayman, D. Miner, C. H. Terrell, J. R. Sherbotie, J. W. Foreman, C. Przysiecki, E. G. Neilson, J. R. Hoyer*

*Corresponding author for this work

Research output: Contribution to journalArticle

368 Scopus citations

Abstract

The majority of human urinary stones are primarily composed of calcium salts. Although normal urine is frequently supersaturated with respect to calcium oxalate, most humans do not form stones. Inhibitors are among the multiple factors that may influence the complex process of urinary stone formation. We have isolated an inhibitor of calcium oxalate crystal growth from human urine by monoclonal antibody immunoaffinity chromatography. The N-terminal amino acid sequence and acidic amino acid content of this aspartic acid-rich protein, uropontin, are similar to those of other pontin proteins from bone, plasma, breast milk, and cells. The inhibitory effect of uropontin on calcium oxalate crystal growth in vitro supports the concept that pontins may have a regulatory role. This function would be analogous to that of other members of the aspartic acid-rich protein superfamily, which stereospecifically regulate the mineralization fronts of calcium-containing crystals. (.

Original languageEnglish (US)
Pages (from-to)426-430
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number1
DOIs
StatePublished - 1992

Keywords

  • Biomineralization
  • Osteopontin

ASJC Scopus subject areas

  • General

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    Shiraga, H., Min, W., Vandusen, W. J., Clayman, M. D., Miner, D., Terrell, C. H., Sherbotie, J. R., Foreman, J. W., Przysiecki, C., Neilson, E. G., & Hoyer, J. R. (1992). Inhibition of calcium oxalate crystal growth in vitro by uropontin: Another member of the aspartic acid-rich protein superfamily. Proceedings of the National Academy of Sciences of the United States of America, 89(1), 426-430. https://doi.org/10.1073/pnas.89.1.426