TY - JOUR
T1 - Inhibition of human polymorphonuclear leukocyte chemotaxis by oxygenated sterol compounds
AU - Gordon, L. I.
AU - Bass, J.
AU - Yachnin, S.
PY - 1980
Y1 - 1980
N2 - When preincubated with certain oxygenated sterol compounds in lipoprotein-depleted serum [20% (vol/vol)], human polymorphonuclear leukocytes show inhibition of chemotaxis toward the synthetic dipeptide N-formylmethionyl-phenylalanine without alteration of random movement or loss of cell viability. These effects can occur at sterol concentrations as low as 6.25 μM and after as little as 5 min of preincubation, but they are increased at higher concentrations and longer preincubation times. The inhibition can be almost completely reversed by preincubation in lipoprotein-replete serum [human AB serum, 20% (vol/vol)] and may be partially corrected by addition of free cholesterol (0.125 mM) to the medium. These effects are unlikely to be due to inhibition of cellular sterol synthesis, competition for chemotaxin membrane binding sites, or deactivation of the leukocytes but they may be a consequence of insertion of the sterol molecule into the leukocyte plasma membranes.
AB - When preincubated with certain oxygenated sterol compounds in lipoprotein-depleted serum [20% (vol/vol)], human polymorphonuclear leukocytes show inhibition of chemotaxis toward the synthetic dipeptide N-formylmethionyl-phenylalanine without alteration of random movement or loss of cell viability. These effects can occur at sterol concentrations as low as 6.25 μM and after as little as 5 min of preincubation, but they are increased at higher concentrations and longer preincubation times. The inhibition can be almost completely reversed by preincubation in lipoprotein-replete serum [human AB serum, 20% (vol/vol)] and may be partially corrected by addition of free cholesterol (0.125 mM) to the medium. These effects are unlikely to be due to inhibition of cellular sterol synthesis, competition for chemotaxin membrane binding sites, or deactivation of the leukocytes but they may be a consequence of insertion of the sterol molecule into the leukocyte plasma membranes.
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U2 - 10.1073/pnas.77.7.4313
DO - 10.1073/pnas.77.7.4313
M3 - Article
C2 - 6933483
AN - SCOPUS:0019309816
VL - 77
SP - 4313
EP - 4316
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 7 II
ER -