Inhibition of p53 acetylation by INHAT subunit SET/TAF-Iβ represses p53 activity

Ji Young Kim, Kyu Sun Lee, Jin Ee Seol, Kweon Yu, Debabrata Chakravarti, Sang Beom Seo*

*Corresponding author for this work

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The tumor suppressor p53 responds to a wide variety of cellular stress signals. Among potential regulatory pathways, post-translational modifications such as acetylation by CBP/p300 and PCAF have been suggested for modulation of p53 activity. However, exactly how p53 acetylation is modulated remains poorly understood. Here, we found that SET/TAF-Iβ inhibited p300-and PCAF-mediated p53 acetylation in an INHAT (inhibitor of histone acetyltransferase) domain-dependent manner. SET/TAF-Iβ interacted with p53 and repressed transcription of p53 target genes. Consequently, SET/TAF-Iβ blocked both p53-mediated cell cycle arrest and apoptosis in response to cellular stress. Using different apoptosis analyses, including FACS, TUNEL and BrdU incorporation assays, we also found that SET/TAF-Iβ induced cellular proliferation via inhibition of p53 acetylation. Furthermore, we observed that apoptotic Drosophila eye phenotype induced by either dp53 overexpression or UV irradiation was rescued by expression of dSet. Inhibition of dp53 acetylation by dSet was observed in both cases. Our findings provide new insights into the regulation of stress-induced p53 activation by HAT-inhibiting histone chaperone SET/TAF-Iβ.

Original languageEnglish (US)
Pages (from-to)75-87
Number of pages13
JournalNucleic acids research
Volume40
Issue number1
DOIs
StatePublished - Jan 2012

ASJC Scopus subject areas

  • Genetics

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