Inhibition of polymorphonuclear leukocyte oxidative metabolism by exogenous phospholipase C

Leo I. Gordon*, Cynthia Schmeichel, Sheila Prachand, Sigmund A. Weitzman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We studied the effects of exogenous, purified phospholipase C (PLC) on neutrophil oxidative metabolism, lysosomal enzyme release and aggregation. We found that PLC inhibited O2- and H2O2 generation and oxygen consumption, but did not alter glucose oxidation via the hexose monophosphate shunt. In contrast, we found a striking stimulation of aggregation and release of the lysosomal enzymes lysozyme and β-glucuronidase. In experiments designed to further characterize the mechanism of the PLC effect on membrane activation we studied the effect of PLC on intracellular calcium concentration [Ca2+]i and found that PLC did not interfere with the fMLP-mediated rise in [Ca2+]i, suggesting that its inhibitory effect on the respiratory burst does not involve inhibition of early signal transduction events. In addition, we found that PLC alone results in mobilization of intracellular Ca2+ stores, consistent with its stimulatory effect on aggregation and lysosomal enzyme release.

Original languageEnglish (US)
Pages (from-to)503-515
Number of pages13
JournalCellular Immunology
Volume128
Issue number2
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Immunology

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