Inhibition of trifluoperazine-induced DNA fragmentation by cyclic AMP mediated signaling

Ung Gu Kang; Myung Jong Kim; Pann Ghill Suh; Sung Ho Ryu; Joo Bae Park; Jung Hye Kim; Yong Sik Kim; Young Han Lee

Inhibition of trifluoperazine-induced DNA fragmentation by cyclic AMP mediated signaling

Ung Gu Kang, Myung Jong Kim, Pann Ghill Suh, Sung Ho Ryu, Joo Bae Park, Jung Hye Kim, Yong Sik Kim, Young Han Lee^*

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Trifluoperazine (TFP), a phenothiazine antipsychotic agent with calmodulin antagonist property, induces DNA fragmentation in a dose- and time-dependent manner in PC12 cells. Various agents affecting calcium mediated intracellular signal transduction such as calcium chelators, calcium ionopores, inhibitors of phospholipase C, and activators/inhibitors of protein kinase C did not block TFP-induced DNA fragmentation. Some of these agents themselves induced DNA fragmentation in the conditions under which they were examined. However, cholera toxin (selective Gs activator), forskolin (adenylate cyclase activator) or dibutyryl cyclic AMP (cyclic AMP analogue) inhibited TFP-induced DNA fragmentation in a dose-dependent manner. These results suggest that it is not the calcium but the Gs and adenylate cyclase pathways that play an important role in TFP-induced DNA fragmentation in PC12 cells.

Original language	English (US)
Pages (from-to)	596-602
Number of pages	7
Journal	Molecules and Cells
Volume	9
Issue number	6
State	Published - Dec 31 1999

Keywords

Calcium
Calmodulin
Cyclic AMP
DNA Fragmentation
PC12 Cell
Trifluoperazine

ASJC Scopus subject areas

Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{de5577b0f9ea4a9aae231178787d647a,

title = "Inhibition of trifluoperazine-induced DNA fragmentation by cyclic AMP mediated signaling",

abstract = "Trifluoperazine (TFP), a phenothiazine antipsychotic agent with calmodulin antagonist property, induces DNA fragmentation in a dose- and time-dependent manner in PC12 cells. Various agents affecting calcium mediated intracellular signal transduction such as calcium chelators, calcium ionopores, inhibitors of phospholipase C, and activators/inhibitors of protein kinase C did not block TFP-induced DNA fragmentation. Some of these agents themselves induced DNA fragmentation in the conditions under which they were examined. However, cholera toxin (selective Gs activator), forskolin (adenylate cyclase activator) or dibutyryl cyclic AMP (cyclic AMP analogue) inhibited TFP-induced DNA fragmentation in a dose-dependent manner. These results suggest that it is not the calcium but the Gs and adenylate cyclase pathways that play an important role in TFP-induced DNA fragmentation in PC12 cells.",

keywords = "Calcium, Calmodulin, Cyclic AMP, DNA Fragmentation, PC12 Cell, Trifluoperazine",

author = "Kang, {Ung Gu} and Kim, {Myung Jong} and Suh, {Pann Ghill} and Ryu, {Sung Ho} and Park, {Joo Bae} and Kim, {Jung Hye} and Kim, {Yong Sik} and Lee, {Young Han}",

year = "1999",

month = dec,

day = "31",

language = "English (US)",

volume = "9",

pages = "596--602",

journal = "Molecules and Cells",

issn = "1016-8478",

publisher = "Korean Society for Molecular and Cellular Biology",

number = "6",

}

TY - JOUR

T1 - Inhibition of trifluoperazine-induced DNA fragmentation by cyclic AMP mediated signaling

AU - Kang, Ung Gu

AU - Kim, Myung Jong

AU - Suh, Pann Ghill

AU - Ryu, Sung Ho

AU - Park, Joo Bae

AU - Kim, Jung Hye

AU - Kim, Yong Sik

AU - Lee, Young Han

PY - 1999/12/31

Y1 - 1999/12/31

N2 - Trifluoperazine (TFP), a phenothiazine antipsychotic agent with calmodulin antagonist property, induces DNA fragmentation in a dose- and time-dependent manner in PC12 cells. Various agents affecting calcium mediated intracellular signal transduction such as calcium chelators, calcium ionopores, inhibitors of phospholipase C, and activators/inhibitors of protein kinase C did not block TFP-induced DNA fragmentation. Some of these agents themselves induced DNA fragmentation in the conditions under which they were examined. However, cholera toxin (selective Gs activator), forskolin (adenylate cyclase activator) or dibutyryl cyclic AMP (cyclic AMP analogue) inhibited TFP-induced DNA fragmentation in a dose-dependent manner. These results suggest that it is not the calcium but the Gs and adenylate cyclase pathways that play an important role in TFP-induced DNA fragmentation in PC12 cells.

AB - Trifluoperazine (TFP), a phenothiazine antipsychotic agent with calmodulin antagonist property, induces DNA fragmentation in a dose- and time-dependent manner in PC12 cells. Various agents affecting calcium mediated intracellular signal transduction such as calcium chelators, calcium ionopores, inhibitors of phospholipase C, and activators/inhibitors of protein kinase C did not block TFP-induced DNA fragmentation. Some of these agents themselves induced DNA fragmentation in the conditions under which they were examined. However, cholera toxin (selective Gs activator), forskolin (adenylate cyclase activator) or dibutyryl cyclic AMP (cyclic AMP analogue) inhibited TFP-induced DNA fragmentation in a dose-dependent manner. These results suggest that it is not the calcium but the Gs and adenylate cyclase pathways that play an important role in TFP-induced DNA fragmentation in PC12 cells.

KW - Calcium

KW - Calmodulin

KW - Cyclic AMP

KW - DNA Fragmentation

KW - PC12 Cell

KW - Trifluoperazine

UR - http://www.scopus.com/inward/record.url?scp=0033621288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033621288&partnerID=8YFLogxK

M3 - Article

C2 - 10672925

AN - SCOPUS:0033621288

SN - 1016-8478

VL - 9

SP - 596

EP - 602

JO - Molecules and Cells

JF - Molecules and Cells

IS - 6

ER -

Inhibition of trifluoperazine-induced DNA fragmentation by cyclic AMP mediated signaling

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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