Inhibitory effect of polyactin A on the DNA, RNA and protein synthesis of tumor cells in vitro

W. Qiang*, J. Liu, Y. Wang, Y. Lei

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Polyactin A(PAA), a new home-made biological response modifier, had various biological activities. The prescursors of radioactivity uptake assay, which measure the incorporation of 3H-TdR or 3H-UR or 3H-Leu into DNA, RNA or protein by surviving cells, were used to study the effect of PAA on K562, HL-60, E4B11C9 hybridoma and B16-F10 melanoma cell in vitro. It was found that PAA ranged from 10 to 5000 μg/ml could inhibit the DNA, RNA and protein synthesis in the K562, HL-60, E4B11C9 hybridoma and B16-F10 melanoma cell at different degree. Especially, the RNA synthesis was strong inhibited at large dose. The effect of PAA was not only dose-dependent, but also different from the cell lines. These results suggest that PAA possessed the inhibitory actions of cell growth, which may play an important role in antitumor activity.

Original languageEnglish (US)
Pages (from-to)230-233
Number of pages4
JournalChinese Journal of Antibiotics
Volume19
Issue number3
StatePublished - Jan 1 1994

Keywords

  • anti-tumor activity
  • biological response modifier
  • polyactin A
  • the precursor of radioactivity incorporation assay

ASJC Scopus subject areas

  • Pharmacology

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