Inhibitory molecules expressed in T lymphocytes in patients with lung cancer before and after chemotherapy

Yujie Xu, Yi Zhang, Xiankui Kuang, Xiufang Duan, Jieyao Li, Jianbin Li, Zhen Zhang, Bin Zhang, Liping Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Objective: The tumongenesis, progression, and metastasis of lung cancer are mostly governed by the immunosuppressive profile. This study aimed to explore the levels of various immunosuppressive inhibitory molecules in lung-cancer patients subjected to different chemotherapy cycles. Methods: Thirty-three patients with advanced lung cancer (ALC; stages III-IV) without receiving prior chemotherapy and 23 healthy subjects were enrolled in our study. Peripheral blood samples were collected from the patients before each chemotherapy cycle. The inhibitory markers expressed in T cells such as TIM3, PD-1, and CTLA4 were analyzed by flow cytometry. Results: The percentages of CD4+ T1M3+, CD8+ TIM3\ CD4+ PD-1+, CD8+ PD-1+, CD4+ CTLA-4+, and CD8+ CTLA-4+ T cells in the peripheral blood of the ALC patients were significantly higher compared to the controls. The percentage of CD4+ TIM3+, CD8+ TIM3+, CD4 + PD-1+, and CD8+ PD-1+ T lymphocytes in the peripheral blood of patients (n=19) who achieved PR or SD significantly decreased after five cycles of chemotherapy (PO.05). Similarly, the percentages of CD4+ CTLA-4+ and CD8 +CTLA-4+ T cells in the patients also decreased after five cycles of treatment. Conclusion: The immune status of ALC patients was evidently suppressed. Effective chemotherapy successfully potentiated effective immune responses by downregulating inhibitory molecules in T cells.

Original languageEnglish (US)
Pages (from-to)960-964
Number of pages5
JournalChinese Journal of Clinical Oncology
Volume40
Issue number16
DOIs
StatePublished - Aug 30 2013

Keywords

  • Advanced lung cancer
  • Chemotherapy
  • Flow cytometry
  • Inhibitory molecule
  • T cell

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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