TY - JOUR
T1 - Initial validation of a novel protein biomarker panel for active pediatric lupus nephritis
AU - Suzuki, Michiko
AU - Wiers, Kristina
AU - Brooks, Elizabeth B.
AU - Greis, Kenneth D.
AU - Haines, Kathleen
AU - Klein-Gitelman, Marisa S.
AU - Olson, Judyann
AU - Onel, Karen
AU - O'Neil, Kathleen M.
AU - Silverman, Earl D.
AU - Tucker, Lori
AU - Ying, Jun
AU - Devarajan, Prasad
AU - Brunner, Hermine I.
PY - 2009/5
Y1 - 2009/5
N2 - Lupus nephritis (LN) is among the main determinants of poor prognosis in systemic lupus erythematosus (SLE). The objective of this study was to 1) isolate and identify proteins contained in the LN urinary protein signature (PS) of children with SLE; 2) assess the usefulness of the PS proteins for detecting activity of LN over time. Using surface-enhanced or matrix-assisted laser desorption/ ionization time of flight mass spectrometry, the proteins contained in the LN urinary PS were identified. They were transferrin (Tf), ceruloplasmin (Cp), α1-acid-glycoprotein (AGP), lipocalin-type prostaglandin-D synthetase (L-PGDS), albumin, and albumin-related fragments. Serial plasma and urine samples were analyzed using immunonephelometry or ELISA in 98 children with SLE (78% African American) and 30 controls with juvenile idiopathic arthritis. All urinary PS proteins were significantly higher with active vs. inactive LN or in patients without LN (all p < 0.005), and their combined area under the receiver operating characteristic curve was 0.85. As early as 3 mo before a clinical diagnosis of worsening LN, significant increases of urinary Tf, AGP (both p < 0.0001), and L-PGDS (p < 0.01) occurred, indicating that these PS proteins are biomarkers of LN activity and may help anticipate the future course of LN.
AB - Lupus nephritis (LN) is among the main determinants of poor prognosis in systemic lupus erythematosus (SLE). The objective of this study was to 1) isolate and identify proteins contained in the LN urinary protein signature (PS) of children with SLE; 2) assess the usefulness of the PS proteins for detecting activity of LN over time. Using surface-enhanced or matrix-assisted laser desorption/ ionization time of flight mass spectrometry, the proteins contained in the LN urinary PS were identified. They were transferrin (Tf), ceruloplasmin (Cp), α1-acid-glycoprotein (AGP), lipocalin-type prostaglandin-D synthetase (L-PGDS), albumin, and albumin-related fragments. Serial plasma and urine samples were analyzed using immunonephelometry or ELISA in 98 children with SLE (78% African American) and 30 controls with juvenile idiopathic arthritis. All urinary PS proteins were significantly higher with active vs. inactive LN or in patients without LN (all p < 0.005), and their combined area under the receiver operating characteristic curve was 0.85. As early as 3 mo before a clinical diagnosis of worsening LN, significant increases of urinary Tf, AGP (both p < 0.0001), and L-PGDS (p < 0.01) occurred, indicating that these PS proteins are biomarkers of LN activity and may help anticipate the future course of LN.
UR - http://www.scopus.com/inward/record.url?scp=69049106426&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69049106426&partnerID=8YFLogxK
U2 - 10.1203/PDR.0b013e31819e4305
DO - 10.1203/PDR.0b013e31819e4305
M3 - Article
C2 - 19218887
AN - SCOPUS:69049106426
SN - 0031-3998
VL - 65
SP - 530
EP - 536
JO - Pediatric research
JF - Pediatric research
IS - 5
ER -