Inositol hexaphosphate inhibits growth and induces differentiation of pc-3 human prostate cancer cells

Abulkalam M. Shamsuddin*, Guang Yu Yang

*Corresponding author for this work

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

We investigated the effects of inositol hexaphosphate (InsP6) on growth inhibition and differentiation of human prostate cancer cells PC-3 in vitro. A significant dose- and time-dependent growth inhibition was observed as tested by the MTT-incorporation assay (P < 0.05 at 1 mM InsP6 after 24 h treatment, P < 0.01 at 0.1 mM after 3 days). DNA synthesis as determined by [3H]thymidine incorporation assay was also suppressed by InsP6 in a dose-dependent manner, occurring as early as 3 h after treatment and continuing up to 48 h (P < 0.01 at 1 mM InsP6). A 9- to 10-fold increase (P < 0.01) in expression of HLA class I molecule associated with tumor immunosurveiUance and cell differentiation was induced by InsP6. The marker for prostatic cell differentiation, prostate acid phosphatase, was significantly (P < 0.05) increased after 48 h treatment at 0.5-5 mM InsP6. Since InsP6 strongly inhibits growth and induces differentiation in human prostate cancer cells in vitro, in vivo studies using a tumor xenograft model and a prostate carcinogenesis model are warranted to validate the efficacy of InsP6 in the treatment and prevention of prostate cancer.

Original languageEnglish (US)
Pages (from-to)1975-1979
Number of pages5
JournalCarcinogenesis
Volume16
Issue number8
DOIs
StatePublished - Aug 1 1995

ASJC Scopus subject areas

  • Cancer Research

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