Insertionally inactivated and inducible recA alleles for use in Neisseria

H. Steven Seifert*

*Corresponding author for this work

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec- and Rec+ by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable cassettes can also be used to control the expression of any chromosomal gene.

Original languageEnglish (US)
Pages (from-to)215-220
Number of pages6
JournalGene
Volume188
Issue number2
DOIs
StatePublished - Apr 1 1997

Keywords

  • DNA recombination
  • DNA repair
  • DNA transformation
  • Pilin variation
  • lac operon

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Insertionally inactivated and inducible recA alleles for use in Neisseria'. Together they form a unique fingerprint.

  • Cite this