Insm1 promotes neurogenic proliferation in delaminated otic progenitors

Sarah M. Lorenzen, Anne Duggan, Anna B. Osipovich, Mark A. Magnuson, Jaime Garcia-Anoveros*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

INSM1 is a zinc-finger protein expressed throughout the developing nervous system in late neuronal progenitors and nascent neurons. In the embryonic cortex and olfactory epithelium, Insm1 may promote the transition of progenitors from apical, proliferative, and uncommitted to basal, terminally-dividing and neuron producing. In the otocyst, delaminating and delaminated progenitors express Insm1, whereas apically-dividing progenitors do not. This expression pattern is analogous to that in embryonic olfactory epithelium and cortex (basal/subventricular progenitors). Lineage analysis confirms that auditory and vestibular neurons originate from Insm1-expressing cells. In the absence of Insm1, otic ganglia are smaller, with 40% fewer neurons. Accounting for the decrease in neurons, delaminated progenitors undergo fewer mitoses, but there is no change in apoptosis. We conclude that in the embryonic inner ear, Insm1 promotes proliferation of delaminated neuronal progenitors and hence the production of neurons, a similar function to that in other embryonic neural epithelia. Unexpectedly, we also found that differentiating, but not mature, outer hair cells express Insm1, whereas inner hair cells do not. Insm1 is the earliest known gene expressed in outer versus inner hair cells, demonstrating that nascent outer hair cells initiate a unique differentiation program in the embryo, much earlier than previously believed.

Original languageEnglish (US)
Pages (from-to)233-245
Number of pages13
JournalMechanisms of Development
Volume138
DOIs
StatePublished - Nov 1 2015

Fingerprint

Ear
Outer Auditory Hair Cells
Neurons
Inner Auditory Hair Cells
Olfactory Mucosa
Parasympathetic Ganglia
Zinc Fingers
Inner Ear
Mitosis
Nervous System
Embryonic Structures
Epithelium
Apoptosis
Genes
Proteins

Keywords

  • Basal progenitors
  • Otocyst
  • Outer hair cell
  • Spiral ganglion
  • Subventricular zone
  • Vestibular ganglion

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Lorenzen, Sarah M. ; Duggan, Anne ; Osipovich, Anna B. ; Magnuson, Mark A. ; Garcia-Anoveros, Jaime. / Insm1 promotes neurogenic proliferation in delaminated otic progenitors. In: Mechanisms of Development. 2015 ; Vol. 138. pp. 233-245.
@article{8716b067b2794a119dbf3e96dce9f5f2,
title = "Insm1 promotes neurogenic proliferation in delaminated otic progenitors",
abstract = "INSM1 is a zinc-finger protein expressed throughout the developing nervous system in late neuronal progenitors and nascent neurons. In the embryonic cortex and olfactory epithelium, Insm1 may promote the transition of progenitors from apical, proliferative, and uncommitted to basal, terminally-dividing and neuron producing. In the otocyst, delaminating and delaminated progenitors express Insm1, whereas apically-dividing progenitors do not. This expression pattern is analogous to that in embryonic olfactory epithelium and cortex (basal/subventricular progenitors). Lineage analysis confirms that auditory and vestibular neurons originate from Insm1-expressing cells. In the absence of Insm1, otic ganglia are smaller, with 40{\%} fewer neurons. Accounting for the decrease in neurons, delaminated progenitors undergo fewer mitoses, but there is no change in apoptosis. We conclude that in the embryonic inner ear, Insm1 promotes proliferation of delaminated neuronal progenitors and hence the production of neurons, a similar function to that in other embryonic neural epithelia. Unexpectedly, we also found that differentiating, but not mature, outer hair cells express Insm1, whereas inner hair cells do not. Insm1 is the earliest known gene expressed in outer versus inner hair cells, demonstrating that nascent outer hair cells initiate a unique differentiation program in the embryo, much earlier than previously believed.",
keywords = "Basal progenitors, Otocyst, Outer hair cell, Spiral ganglion, Subventricular zone, Vestibular ganglion",
author = "Lorenzen, {Sarah M.} and Anne Duggan and Osipovich, {Anna B.} and Magnuson, {Mark A.} and Jaime Garcia-Anoveros",
year = "2015",
month = "11",
day = "1",
doi = "10.1016/j.mod.2015.11.001",
language = "English (US)",
volume = "138",
pages = "233--245",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier Ireland Ltd",

}

Insm1 promotes neurogenic proliferation in delaminated otic progenitors. / Lorenzen, Sarah M.; Duggan, Anne; Osipovich, Anna B.; Magnuson, Mark A.; Garcia-Anoveros, Jaime.

In: Mechanisms of Development, Vol. 138, 01.11.2015, p. 233-245.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Insm1 promotes neurogenic proliferation in delaminated otic progenitors

AU - Lorenzen, Sarah M.

AU - Duggan, Anne

AU - Osipovich, Anna B.

AU - Magnuson, Mark A.

AU - Garcia-Anoveros, Jaime

PY - 2015/11/1

Y1 - 2015/11/1

N2 - INSM1 is a zinc-finger protein expressed throughout the developing nervous system in late neuronal progenitors and nascent neurons. In the embryonic cortex and olfactory epithelium, Insm1 may promote the transition of progenitors from apical, proliferative, and uncommitted to basal, terminally-dividing and neuron producing. In the otocyst, delaminating and delaminated progenitors express Insm1, whereas apically-dividing progenitors do not. This expression pattern is analogous to that in embryonic olfactory epithelium and cortex (basal/subventricular progenitors). Lineage analysis confirms that auditory and vestibular neurons originate from Insm1-expressing cells. In the absence of Insm1, otic ganglia are smaller, with 40% fewer neurons. Accounting for the decrease in neurons, delaminated progenitors undergo fewer mitoses, but there is no change in apoptosis. We conclude that in the embryonic inner ear, Insm1 promotes proliferation of delaminated neuronal progenitors and hence the production of neurons, a similar function to that in other embryonic neural epithelia. Unexpectedly, we also found that differentiating, but not mature, outer hair cells express Insm1, whereas inner hair cells do not. Insm1 is the earliest known gene expressed in outer versus inner hair cells, demonstrating that nascent outer hair cells initiate a unique differentiation program in the embryo, much earlier than previously believed.

AB - INSM1 is a zinc-finger protein expressed throughout the developing nervous system in late neuronal progenitors and nascent neurons. In the embryonic cortex and olfactory epithelium, Insm1 may promote the transition of progenitors from apical, proliferative, and uncommitted to basal, terminally-dividing and neuron producing. In the otocyst, delaminating and delaminated progenitors express Insm1, whereas apically-dividing progenitors do not. This expression pattern is analogous to that in embryonic olfactory epithelium and cortex (basal/subventricular progenitors). Lineage analysis confirms that auditory and vestibular neurons originate from Insm1-expressing cells. In the absence of Insm1, otic ganglia are smaller, with 40% fewer neurons. Accounting for the decrease in neurons, delaminated progenitors undergo fewer mitoses, but there is no change in apoptosis. We conclude that in the embryonic inner ear, Insm1 promotes proliferation of delaminated neuronal progenitors and hence the production of neurons, a similar function to that in other embryonic neural epithelia. Unexpectedly, we also found that differentiating, but not mature, outer hair cells express Insm1, whereas inner hair cells do not. Insm1 is the earliest known gene expressed in outer versus inner hair cells, demonstrating that nascent outer hair cells initiate a unique differentiation program in the embryo, much earlier than previously believed.

KW - Basal progenitors

KW - Otocyst

KW - Outer hair cell

KW - Spiral ganglion

KW - Subventricular zone

KW - Vestibular ganglion

UR - http://www.scopus.com/inward/record.url?scp=84949232524&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949232524&partnerID=8YFLogxK

U2 - 10.1016/j.mod.2015.11.001

DO - 10.1016/j.mod.2015.11.001

M3 - Article

VL - 138

SP - 233

EP - 245

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

ER -