Intact B-cell activity is essential for complete expression of experimental allergic encephalomyelitis in Lewis rats

J. Gausas; P. Y. Paterson; E. D. Day; M. C. Dal Canto

doi:10.1016/0008-8749(82)90484-1

Intact B-cell activity is essential for complete expression of experimental allergic encephalomyelitis in Lewis rats

J. Gausas, P. Y. Paterson^*, E. D. Day, M. C. Dal Canto

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

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Abstract

B-Cell activity in weanling Lewis rats was inhibited by injections of goat anti-rat mu-chain antiserum (GAMS) from birth on, as demonstrated by markedly reduced or absent myelin basic protein (MBP) binding activity of sera. Following challenge with guinea pig spinal cord (GPSC)-complete Freund's adjuvant (CFA) and continued GAMS treatment, all rats remained clinically well without a trace of neurological signs. Histopathologically, central nervous system (CNS) perivascular fibrin deposits, typically accompanying paralytic signs of the disease, were virtually absent in GAMS-treated rats, despite occurrence of perivascular cell infiltrates of variable degrees of severity. In addition, preliminary studies of plastic embedded tissues suggest that demyelination is appreciably reduced in GAMS-treated and challenged rats. In contrast, control littermates similarly treated with normal goat serum (NGS) or normal goat IgG (NGIgG) and challenged, developed typical paralytic signs and perivascular fibrin deposits characteristic focal perivascular cellular infiltrates and demyelination of EAE, as well as anti-MBP antibodies.

Original language	English (US)
Pages (from-to)	360-366
Number of pages	7
Journal	Cellular Immunology
Volume	72
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(82)90484-1
State	Published - Sep 15 1982

ASJC Scopus subject areas

Immunology

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@article{1e8293028a7946f6a71784752c3a0743,

title = "Intact B-cell activity is essential for complete expression of experimental allergic encephalomyelitis in Lewis rats",

abstract = "B-Cell activity in weanling Lewis rats was inhibited by injections of goat anti-rat mu-chain antiserum (GAMS) from birth on, as demonstrated by markedly reduced or absent myelin basic protein (MBP) binding activity of sera. Following challenge with guinea pig spinal cord (GPSC)-complete Freund's adjuvant (CFA) and continued GAMS treatment, all rats remained clinically well without a trace of neurological signs. Histopathologically, central nervous system (CNS) perivascular fibrin deposits, typically accompanying paralytic signs of the disease, were virtually absent in GAMS-treated rats, despite occurrence of perivascular cell infiltrates of variable degrees of severity. In addition, preliminary studies of plastic embedded tissues suggest that demyelination is appreciably reduced in GAMS-treated and challenged rats. In contrast, control littermates similarly treated with normal goat serum (NGS) or normal goat IgG (NGIgG) and challenged, developed typical paralytic signs and perivascular fibrin deposits characteristic focal perivascular cellular infiltrates and demyelination of EAE, as well as anti-MBP antibodies.",

author = "J. Gausas and Paterson, {P. Y.} and Day, {E. D.} and {Dal Canto}, {M. C.}",

note = "Funding Information: This work supported in part at Northwestern University by U.S. Public Health Service research Grants NS 06262 and NS-1301 1, and at Duke University by research Grants 833-3-5 from the National Multiple Sclerosis Society and NS-10237 from the National Institutes of Health of the U.S. Public Health Service. The authors wish to acknowledge the invaluable technical assistance of Mrs. C. Clark, Mm.. C. Walker, and Miss J. Tong. Mrs. Geane Kraus and Mrs. Sandy Horeis provided superb assistance in the preparation of this manuscript.",

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doi = "10.1016/0008-8749(82)90484-1",

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AU - Gausas, J.

AU - Paterson, P. Y.

AU - Day, E. D.

AU - Dal Canto, M. C.

N1 - Funding Information: This work supported in part at Northwestern University by U.S. Public Health Service research Grants NS 06262 and NS-1301 1, and at Duke University by research Grants 833-3-5 from the National Multiple Sclerosis Society and NS-10237 from the National Institutes of Health of the U.S. Public Health Service. The authors wish to acknowledge the invaluable technical assistance of Mrs. C. Clark, Mm.. C. Walker, and Miss J. Tong. Mrs. Geane Kraus and Mrs. Sandy Horeis provided superb assistance in the preparation of this manuscript.

PY - 1982/9/15

Y1 - 1982/9/15

N2 - B-Cell activity in weanling Lewis rats was inhibited by injections of goat anti-rat mu-chain antiserum (GAMS) from birth on, as demonstrated by markedly reduced or absent myelin basic protein (MBP) binding activity of sera. Following challenge with guinea pig spinal cord (GPSC)-complete Freund's adjuvant (CFA) and continued GAMS treatment, all rats remained clinically well without a trace of neurological signs. Histopathologically, central nervous system (CNS) perivascular fibrin deposits, typically accompanying paralytic signs of the disease, were virtually absent in GAMS-treated rats, despite occurrence of perivascular cell infiltrates of variable degrees of severity. In addition, preliminary studies of plastic embedded tissues suggest that demyelination is appreciably reduced in GAMS-treated and challenged rats. In contrast, control littermates similarly treated with normal goat serum (NGS) or normal goat IgG (NGIgG) and challenged, developed typical paralytic signs and perivascular fibrin deposits characteristic focal perivascular cellular infiltrates and demyelination of EAE, as well as anti-MBP antibodies.

AB - B-Cell activity in weanling Lewis rats was inhibited by injections of goat anti-rat mu-chain antiserum (GAMS) from birth on, as demonstrated by markedly reduced or absent myelin basic protein (MBP) binding activity of sera. Following challenge with guinea pig spinal cord (GPSC)-complete Freund's adjuvant (CFA) and continued GAMS treatment, all rats remained clinically well without a trace of neurological signs. Histopathologically, central nervous system (CNS) perivascular fibrin deposits, typically accompanying paralytic signs of the disease, were virtually absent in GAMS-treated rats, despite occurrence of perivascular cell infiltrates of variable degrees of severity. In addition, preliminary studies of plastic embedded tissues suggest that demyelination is appreciably reduced in GAMS-treated and challenged rats. In contrast, control littermates similarly treated with normal goat serum (NGS) or normal goat IgG (NGIgG) and challenged, developed typical paralytic signs and perivascular fibrin deposits characteristic focal perivascular cellular infiltrates and demyelination of EAE, as well as anti-MBP antibodies.

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Intact B-cell activity is essential for complete expression of experimental allergic encephalomyelitis in Lewis rats

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