Integrated multimodal genetic testing of Ewing sarcoma - A single-institution experience

Mikako Warren, Michael Weindel, Jo Ringrose, Clint Venable, Adriana Reyes, Keita Terashima, Pulivarthi Rao, Murali Chintagumpala, M. John Hicks, Dolores Lopez-Terrada, Xin Yan Lu*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Summary Ewing sarcoma (ES) is an aggressive malignant small round cell tumor that arises in bone or soft tissue of adolescents and young adults. A characteristic molecular finding in ES is EWSR1 gene fusion with ETS (erythroblast transformation-specific) family genes including FLI1 (~90%) and ERG (>5%). Here we report our experience using integrated clinicopathologic, cytogenetic, fluorescence in situ hybridization (FISH), and reverse transcriptase polymerase chain reaction (RT-PCR) analyses of 32 pediatric patients with ES diagnosed in a single institution between 2005 and 2011. Diagnostic EWSR1 rearrangements were detected in 30 (93.8%) of 32 patients. Cytogenetics detected t(11;22) (n = 14) and t(21;22) (n = 1) in 15 (46.9%) patients. FISH detected EWSR1 rearrangements in 27 (96.4%) of 28 patients tested. RT-PCR was positive in 27 (84.4%) of 32 patients, including 24 EWSR1-FLI1 and 3 EWSR1-ERG. RT-PCR defined breakpoints and fusion partners in 7 cases with EWSR1 rearrangements detected by FISH. Sanger sequencing further delineated breakpoints in 21 (77.8%) of 27 RT-PCR positive cases. In summary, conventional cytogenetic analysis provided a global view but had a lower detection rate and longer turnaround time than other methods. FISH is a rapid method and theoretically can detect all EWSR1 rearrangements, but it cannot identify all partners and is not completely specific for ES. RT-PCR and sequencing are more sensitive and useful in identifying fusion partners and refining breakpoints; however, these methods can be compromised by poor RNA preservation and primer design. In conclusion, an integrated approach that uses all methods capable of detecting EWSR1 rearrangements has value in the workup of suspected cases of ES.

Original languageEnglish (US)
Pages (from-to)2010-2019
Number of pages10
JournalHuman Pathology
Volume44
Issue number10
DOIs
StatePublished - Oct 1 2013

Keywords

  • Chromosome analysis
  • Ewing sarcoma
  • FISH
  • Integrated testing
  • RT-PCR
  • Sequencing

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Warren, M., Weindel, M., Ringrose, J., Venable, C., Reyes, A., Terashima, K., Rao, P., Chintagumpala, M., Hicks, M. J., Lopez-Terrada, D., & Lu, X. Y. (2013). Integrated multimodal genetic testing of Ewing sarcoma - A single-institution experience. Human Pathology, 44(10), 2010-2019. https://doi.org/10.1016/j.humpath.2013.03.003