Integration of Genetic and Cytological Maps and Development of a Pachytene Chromosome-based Karyotype in Papaya

Wenli Zhang, Ching Man Wai, Ray Ming, Qingyi Yu, Jiming Jiang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

A significant amount of genetic and genomic resources have been developed in papaya (Carica papaya, 2n = 2 × = 18), including genetic linkage maps consisting of nine major and three minor linkage groups. However, the 12 genetic linkage groups have not been integrated with the nine chromosomes of papaya. Bacterial artificial chromosome (BAC) clones associated with each linkage group were recently isolated. These linkage group-specific BACs were mapped to meiotic pachytene chromosomes of papaya using fluorescence in situ hybridization (FISH). The FISH mapping results integrated the 12 linkage groups into the nine papaya chromosomes. We developed a pachytene chromosome-based high resolution karyotype for the hermaphrodite plant genome of papaya cultivar SunUp. The chromosomal distribution of heterochromatin in the papaya genome is provided in the karyotype with the X chromosome representing the most euchromatic chromosome in the papaya genome. FISH mapping also revealed a significant amplification of sequences related to the 5S ribosomal RNA genes, which was detected in the malespecific region of the Y chromosome, but not in the corresponding region in the X chromosome.

Original languageEnglish (US)
Pages (from-to)166-170
Number of pages5
JournalTropical Plant Biology
Volume3
Issue number3
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Carica
  • Chromosome map
  • FISH
  • Karyotype

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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