Interaction of steroidal alkaloid toxins with calcium channels in neuronal cell lines

Sathapana Kongsamut; Stephen B. Freedman; Bradley E. Simon; Richard J. Miller

doi:10.1016/0024-3205(85)90058-X

Interaction of steroidal alkaloid toxins with calcium channels in neuronal cell lines

Sathapana Kongsamut^*, Stephen B. Freedman, Bradley E. Simon, Richard J. Miller

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Depolarization with 50 mM K⁺ increased ⁴⁵Ca²⁺ uptake into neuronal clonal cell lines NG108-15, NIE-115 and NH15-CA2. In each cell line this depolarization-induced uptake was blocked by inorganic and organic blockers of voltage sensitive calcium channels. However, tetrodotoxin (10^-6M) was ineffective. Moreover, in the presence of tetrodotoxin, neither batrachotoxin nor veratridine inhibited the depolarization-induced uptake. The novel dihydropyridine BAY K8644 enhanced depolarization-induced ⁴⁵Ca²⁺ uptake into each cell line in a nitrendipine reversible fashion. In the presence of tetrodotoxin, the BAY K8644/50 mM K⁺ stimulated uptake could be partially inhibited by batrachotoxin (10^-6M) and veratridine (5 x 10^-5 M). These effects were not altered by the presence of scorpion venom (1 μg/ml). The results indicate that both batrachotoxin and veratridine can modulate the affects of dihydropyridines on the gating properties of voltage sensitive calcium channels.

Original language	English (US)
Pages (from-to)	1493-1501
Number of pages	9
Journal	Life Sciences
Volume	36
Issue number	15
DOIs	https://doi.org/10.1016/0024-3205(85)90058-X
State	Published - Apr 15 1985

Funding

The authors would like to thank Drs. Glyn Dawson and Mitchel Villereal for the generous use of their tissue culture facilities, Elisabeth Cornelison for technical assistance and Della Akres for expert preparation of the manuscript. This study was supported by NIH grants DA-02121 and DA-02575 to R.J.M. S.B.F. was a NATO (United Kingdom) Fellow.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.1016/0024-3205(85)90058-X

Cite this

@article{540e2d00bf054634bf1b9af98edcbc4d,

title = "Interaction of steroidal alkaloid toxins with calcium channels in neuronal cell lines",

abstract = "Depolarization with 50 mM K+ increased 45Ca2+ uptake into neuronal clonal cell lines NG108-15, NIE-115 and NH15-CA2. In each cell line this depolarization-induced uptake was blocked by inorganic and organic blockers of voltage sensitive calcium channels. However, tetrodotoxin (10-6M) was ineffective. Moreover, in the presence of tetrodotoxin, neither batrachotoxin nor veratridine inhibited the depolarization-induced uptake. The novel dihydropyridine BAY K8644 enhanced depolarization-induced 45Ca2+ uptake into each cell line in a nitrendipine reversible fashion. In the presence of tetrodotoxin, the BAY K8644/50 mM K+ stimulated uptake could be partially inhibited by batrachotoxin (10-6M) and veratridine (5 x 10-5 M). These effects were not altered by the presence of scorpion venom (1 μg/ml). The results indicate that both batrachotoxin and veratridine can modulate the affects of dihydropyridines on the gating properties of voltage sensitive calcium channels.",

author = "Sathapana Kongsamut and Freedman, {Stephen B.} and Simon, {Bradley E.} and Miller, {Richard J.}",

note = "Funding Information: The authors would like to thank Drs. Glyn Dawson and Mitchel Villereal for the generous use of their tissue culture facilities, Elisabeth Cornelison for technical assistance and Della Akres for expert preparation of the manuscript. This study was supported by NIH grants DA-02121 and DA-02575 to R.J.M. S.B.F. was a NATO (United Kingdom) Fellow.",

year = "1985",

month = apr,

day = "15",

doi = "10.1016/0024-3205(85)90058-X",

language = "English (US)",

volume = "36",

pages = "1493--1501",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "15",

}

TY - JOUR

T1 - Interaction of steroidal alkaloid toxins with calcium channels in neuronal cell lines

AU - Kongsamut, Sathapana

AU - Freedman, Stephen B.

AU - Simon, Bradley E.

AU - Miller, Richard J.

N1 - Funding Information: The authors would like to thank Drs. Glyn Dawson and Mitchel Villereal for the generous use of their tissue culture facilities, Elisabeth Cornelison for technical assistance and Della Akres for expert preparation of the manuscript. This study was supported by NIH grants DA-02121 and DA-02575 to R.J.M. S.B.F. was a NATO (United Kingdom) Fellow.

PY - 1985/4/15

Y1 - 1985/4/15

N2 - Depolarization with 50 mM K+ increased 45Ca2+ uptake into neuronal clonal cell lines NG108-15, NIE-115 and NH15-CA2. In each cell line this depolarization-induced uptake was blocked by inorganic and organic blockers of voltage sensitive calcium channels. However, tetrodotoxin (10-6M) was ineffective. Moreover, in the presence of tetrodotoxin, neither batrachotoxin nor veratridine inhibited the depolarization-induced uptake. The novel dihydropyridine BAY K8644 enhanced depolarization-induced 45Ca2+ uptake into each cell line in a nitrendipine reversible fashion. In the presence of tetrodotoxin, the BAY K8644/50 mM K+ stimulated uptake could be partially inhibited by batrachotoxin (10-6M) and veratridine (5 x 10-5 M). These effects were not altered by the presence of scorpion venom (1 μg/ml). The results indicate that both batrachotoxin and veratridine can modulate the affects of dihydropyridines on the gating properties of voltage sensitive calcium channels.

AB - Depolarization with 50 mM K+ increased 45Ca2+ uptake into neuronal clonal cell lines NG108-15, NIE-115 and NH15-CA2. In each cell line this depolarization-induced uptake was blocked by inorganic and organic blockers of voltage sensitive calcium channels. However, tetrodotoxin (10-6M) was ineffective. Moreover, in the presence of tetrodotoxin, neither batrachotoxin nor veratridine inhibited the depolarization-induced uptake. The novel dihydropyridine BAY K8644 enhanced depolarization-induced 45Ca2+ uptake into each cell line in a nitrendipine reversible fashion. In the presence of tetrodotoxin, the BAY K8644/50 mM K+ stimulated uptake could be partially inhibited by batrachotoxin (10-6M) and veratridine (5 x 10-5 M). These effects were not altered by the presence of scorpion venom (1 μg/ml). The results indicate that both batrachotoxin and veratridine can modulate the affects of dihydropyridines on the gating properties of voltage sensitive calcium channels.

UR - http://www.scopus.com/inward/record.url?scp=0021959174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021959174&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(85)90058-X

DO - 10.1016/0024-3205(85)90058-X

M3 - Article

C2 - 2580208

AN - SCOPUS:0021959174

SN - 0024-3205

VL - 36

SP - 1493

EP - 1501

JO - Life Sciences

JF - Life Sciences

IS - 15

ER -

Interaction of steroidal alkaloid toxins with calcium channels in neuronal cell lines

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this