Interaction of steroidal alkaloid toxins with calcium channels in neuronal cell lines

Sathapana Kongsamut*, Stephen B. Freedman, Bradley E. Simon, Richard J. Miller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Depolarization with 50 mM K+ increased 45Ca2+ uptake into neuronal clonal cell lines NG108-15, NIE-115 and NH15-CA2. In each cell line this depolarization-induced uptake was blocked by inorganic and organic blockers of voltage sensitive calcium channels. However, tetrodotoxin (10-6M) was ineffective. Moreover, in the presence of tetrodotoxin, neither batrachotoxin nor veratridine inhibited the depolarization-induced uptake. The novel dihydropyridine BAY K8644 enhanced depolarization-induced 45Ca2+ uptake into each cell line in a nitrendipine reversible fashion. In the presence of tetrodotoxin, the BAY K8644/50 mM K+ stimulated uptake could be partially inhibited by batrachotoxin (10-6M) and veratridine (5 x 10-5 M). These effects were not altered by the presence of scorpion venom (1 μg/ml). The results indicate that both batrachotoxin and veratridine can modulate the affects of dihydropyridines on the gating properties of voltage sensitive calcium channels.

Original languageEnglish (US)
Pages (from-to)1493-1501
Number of pages9
JournalLife Sciences
Volume36
Issue number15
DOIs
StatePublished - Apr 15 1985

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Interaction of steroidal alkaloid toxins with calcium channels in neuronal cell lines'. Together they form a unique fingerprint.

Cite this