Interaction of steroidal alkaloid toxins with calcium channels in neuronal cell lines

Sathapana Kongsamut*, Stephen B. Freedman, Bradley E. Simon, Richard J. Miller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Depolarization with 50 mM K+ increased 45Ca2+ uptake into neuronal clonal cell lines NG108-15, NIE-115 and NH15-CA2. In each cell line this depolarization-induced uptake was blocked by inorganic and organic blockers of voltage sensitive calcium channels. However, tetrodotoxin (10-6M) was ineffective. Moreover, in the presence of tetrodotoxin, neither batrachotoxin nor veratridine inhibited the depolarization-induced uptake. The novel dihydropyridine BAY K8644 enhanced depolarization-induced 45Ca2+ uptake into each cell line in a nitrendipine reversible fashion. In the presence of tetrodotoxin, the BAY K8644/50 mM K+ stimulated uptake could be partially inhibited by batrachotoxin (10-6M) and veratridine (5 x 10-5 M). These effects were not altered by the presence of scorpion venom (1 μg/ml). The results indicate that both batrachotoxin and veratridine can modulate the affects of dihydropyridines on the gating properties of voltage sensitive calcium channels.

Original languageEnglish (US)
Pages (from-to)1493-1501
Number of pages9
JournalLife Sciences
Volume36
Issue number15
DOIs
StatePublished - Apr 15 1985

Funding

The authors would like to thank Drs. Glyn Dawson and Mitchel Villereal for the generous use of their tissue culture facilities, Elisabeth Cornelison for technical assistance and Della Akres for expert preparation of the manuscript. This study was supported by NIH grants DA-02121 and DA-02575 to R.J.M. S.B.F. was a NATO (United Kingdom) Fellow.

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • Pharmacology, Toxicology and Pharmaceutics(all)

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