Interactions between HIV-1 and Cytomegalovirus in Human Osteosarcoma Cells Carrying Both Viruses

Miriam Margalith, Daniel J. Medina, Gueh Djen Hsiung, Benjamin R. Smith, Richard T. D'aquila, Joan C. Kaplan, Lawrence Bechtel, Monica Z. Wang, Paul R. Skolnik, Martin S. Hirsch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Cytomegalovirus (CMV) and the human immunodeficiency virus type 1 (HIV-1) may interact in the pathogenesis of AIDS. We compared CMV replication in human osteosarcoma (HOS) cells to that in HOS cells genetically engineered to contain an envelope-deficient HIV-1 proviral construct (designated HOS-HXG). Following acute CMV infection of each cell line, HOS-HXG cells contained higher numbers of intranuclear CMV nucleocapsids than did HOS cells. Infectious CMV could be persistently detected in culture supernatant fluids of the CMV-infected HOS-HXG cells, whereas CMV was lost over several weeks from HOS cells infected with CMV in parallel. HIV-1 CMV pseudotypes were not detected in supernatant fluids from CMV-infected HOS-HXG cells. On day 119 after CMV infection, these cultures were superinfected with HIV-1. These dually infected HOS-HXG cells produced infectious HIV-1 and exhibited markedly enhanced CMV replication compared to parental CMV-infected HOS-HXG cells. Two different HIV-1 tat gene function antagonists, Ro24-7429 and chemically modified antibodies to the Tat protein, did not inhibit the replication of CMV in either acute or persistent infections of HOS-HXG cells at concentrations that inhibited HIV-1 replication.

Original languageEnglish (US)
Pages (from-to)519-527
Number of pages9
JournalAIDS research and human retroviruses
Volume9
Issue number6
DOIs
StatePublished - Jun 1993

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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