Interleukin-1β induction of the chemokine RANTES promoter in the human astrocytoma line CH235 requires both constitutive and inducible transcription factors

Neil G. Miyamoto*, Poonam S. Medberry, Joe Hesselgesser, Sabine Boehlk, Peter J. Nelson, Alan M. Krensky, H. Daniel Perez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor requirements for interleukin-1β (IL-1β) induction of the RANTES promoter in the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1β-inducibility. In vitro DNA binding assays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP family members to their cognate sites in the RANTES promoter, whereas NF-κB and IRF-1 bind in an IL-1β-inducible manner. IL-1β-inducibility of the RANTES promoter requires both constitutive and inducible transcription factors. The formation of a higher order nucleoprotein complex, or 'enhanceosome', may be critical for IL-1β induction of the RANTES promoter. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)78-90
Number of pages13
JournalJournal of Neuroimmunology
Volume105
Issue number1
DOIs
StatePublished - Jun 1 2000

Funding

We thank Drs. Ulrike Fuhrmann, Michael Klotzbuecher, and Jacques Ghysdael for gifts of antibodies. We gratefully acknowledge Myrna Faulds for graphics, and Dr. David Asarnow for assistance in cloning. P.J.N. is supported by grants from the DFG Sonderforsungsberich 464 and 469.

Keywords

  • Astrocytes
  • Interleukin-1β
  • Promoter activity
  • RANTES
  • Transcription factors

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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