Interleukin-1β induction of the chemokine RANTES promoter in the human astrocytoma line CH235 requires both constitutive and inducible transcription factors

Neil G. Miyamoto*, Poonam S. Medberry, Joe Hesselgesser, Sabine Boehlk, Peter J. Nelson, Alan M. Krensky, H. Daniel Perez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor requirements for interleukin-1β (IL-1β) induction of the RANTES promoter in the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1β-inducibility. In vitro DNA binding assays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP family members to their cognate sites in the RANTES promoter, whereas NF-κB and IRF-1 bind in an IL-1β-inducible manner. IL-1β-inducibility of the RANTES promoter requires both constitutive and inducible transcription factors. The formation of a higher order nucleoprotein complex, or 'enhanceosome', may be critical for IL-1β induction of the RANTES promoter. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)78-90
Number of pages13
JournalJournal of Neuroimmunology
Volume105
Issue number1
DOIs
StatePublished - Jun 1 2000

Keywords

  • Astrocytes
  • Interleukin-1β
  • Promoter activity
  • RANTES
  • Transcription factors

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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