Interleukin-1β induction of the chemokine RANTES promoter in the human astrocytoma line CH235 requires both constitutive and inducible transcription factors

Neil G. Miyamoto; Poonam S. Medberry; Joe Hesselgesser; Sabine Boehlk; Peter J. Nelson; Alan M. Krensky; H. Daniel Perez

doi:10.1016/S0165-5728(00)00195-8

Interleukin-1β induction of the chemokine RANTES promoter in the human astrocytoma line CH235 requires both constitutive and inducible transcription factors

Neil G. Miyamoto^*, Poonam S. Medberry, Joe Hesselgesser, Sabine Boehlk, Peter J. Nelson, Alan M. Krensky, H. Daniel Perez

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Abstract

The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor requirements for interleukin-1β (IL-1β) induction of the RANTES promoter in the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1β-inducibility. In vitro DNA binding assays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP family members to their cognate sites in the RANTES promoter, whereas NF-κB and IRF-1 bind in an IL-1β-inducible manner. IL-1β-inducibility of the RANTES promoter requires both constitutive and inducible transcription factors. The formation of a higher order nucleoprotein complex, or 'enhanceosome', may be critical for IL-1β induction of the RANTES promoter. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	78-90
Number of pages	13
Journal	Journal of Neuroimmunology
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/S0165-5728(00)00195-8
State	Published - Jun 1 2000

Funding

We thank Drs. Ulrike Fuhrmann, Michael Klotzbuecher, and Jacques Ghysdael for gifts of antibodies. We gratefully acknowledge Myrna Faulds for graphics, and Dr. David Asarnow for assistance in cloning. P.J.N. is supported by grants from the DFG Sonderforsungsberich 464 and 469.

Keywords

Astrocytes
Interleukin-1β
Promoter activity
RANTES
Transcription factors

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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title = "Interleukin-1β induction of the chemokine RANTES promoter in the human astrocytoma line CH235 requires both constitutive and inducible transcription factors",

abstract = "The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor requirements for interleukin-1β (IL-1β) induction of the RANTES promoter in the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1β-inducibility. In vitro DNA binding assays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP family members to their cognate sites in the RANTES promoter, whereas NF-κB and IRF-1 bind in an IL-1β-inducible manner. IL-1β-inducibility of the RANTES promoter requires both constitutive and inducible transcription factors. The formation of a higher order nucleoprotein complex, or 'enhanceosome', may be critical for IL-1β induction of the RANTES promoter. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Astrocytes, Interleukin-1β, Promoter activity, RANTES, Transcription factors",

author = "Miyamoto, \{Neil G.\} and Medberry, \{Poonam S.\} and Joe Hesselgesser and Sabine Boehlk and Nelson, \{Peter J.\} and Krensky, \{Alan M.\} and Perez, \{H. Daniel\}",

note = "Funding Information: We thank Drs. Ulrike Fuhrmann, Michael Klotzbuecher, and Jacques Ghysdael for gifts of antibodies. We gratefully acknowledge Myrna Faulds for graphics, and Dr. David Asarnow for assistance in cloning. P.J.N. is supported by grants from the DFG Sonderforsungsberich 464 and 469.",

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AU - Miyamoto, Neil G.

AU - Medberry, Poonam S.

AU - Hesselgesser, Joe

AU - Boehlk, Sabine

AU - Nelson, Peter J.

AU - Krensky, Alan M.

AU - Perez, H. Daniel

N1 - Funding Information: We thank Drs. Ulrike Fuhrmann, Michael Klotzbuecher, and Jacques Ghysdael for gifts of antibodies. We gratefully acknowledge Myrna Faulds for graphics, and Dr. David Asarnow for assistance in cloning. P.J.N. is supported by grants from the DFG Sonderforsungsberich 464 and 469.

PY - 2000/6/1

Y1 - 2000/6/1

N2 - The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor requirements for interleukin-1β (IL-1β) induction of the RANTES promoter in the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1β-inducibility. In vitro DNA binding assays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP family members to their cognate sites in the RANTES promoter, whereas NF-κB and IRF-1 bind in an IL-1β-inducible manner. IL-1β-inducibility of the RANTES promoter requires both constitutive and inducible transcription factors. The formation of a higher order nucleoprotein complex, or 'enhanceosome', may be critical for IL-1β induction of the RANTES promoter. Copyright (C) 2000 Elsevier Science B.V.

AB - The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor requirements for interleukin-1β (IL-1β) induction of the RANTES promoter in the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1β-inducibility. In vitro DNA binding assays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP family members to their cognate sites in the RANTES promoter, whereas NF-κB and IRF-1 bind in an IL-1β-inducible manner. IL-1β-inducibility of the RANTES promoter requires both constitutive and inducible transcription factors. The formation of a higher order nucleoprotein complex, or 'enhanceosome', may be critical for IL-1β induction of the RANTES promoter. Copyright (C) 2000 Elsevier Science B.V.

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Interleukin-1β induction of the chemokine RANTES promoter in the human astrocytoma line CH235 requires both constitutive and inducible transcription factors

Abstract

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Keywords

ASJC Scopus subject areas

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