Interleukin-12 therapy restores cell-mediated immunity in ethanol- consuming mice

Jeffrey D. Peterson, Kristine Vasquez, Carl Waltenbaugh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Previous studies from our laboratory show that ethanol consumption impairs antigen-specific, cell-mediated, but not, humoral immune responses of C57BL/6, BALB/c, and DO11.10 T-cell receptor transgenic mice. This ethanol- associated deficit is associated with decreased interleukin (IL)-12 and interferon-γ (IFN-γ) production, but not IL-2 or antigen-specific T-cell proliferation by explanted leukocytes from ethanol-consuming mice. IL-12 expression by macrophage/monocytes is viewed as a requirement for the production of IFN-γ by Th1 lymphocytes that mediate cellular immunity. In this study, we restored antigen-specific, cell-mediated immunity, delayed hypersensitivity, to ethanol-consuming C57BL/6 or BALB/c mice with a single 100 ng of intravenous injection of recombinant IL-12 at the time of immunization. The addition of exogenous recombinant IL-12 to co-cultures of antigen-presenting cells derived from ethanol-consuming mice and purified T cells derived from ethanol-nonconsuming DO11.10 repairs the ability of Th1 cells to make IFN-γ in response to antigen. Administration of recombinant IL-12 opens a potential for restoring cell-mediated immune function to ethanol-consuming individuals.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume22
Issue number1
DOIs
StatePublished - Feb 1998

Keywords

  • Alcohol ingestion
  • BALB/c mice
  • C57BL/6 mice
  • Cell-mediated immunity
  • Interleukin-12

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

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