Interleukin-2 in rheumatoid arthritis: Production of and response to interleukin-2 in rheumatoid synovial fluid, synovial tissue and peripheral blood

B. Combe, R. M. Pope, M. Fischbach, B. Darnell, S. Baron, N. Talal

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126 Scopus citations

Abstract

Several aspects of interleukin-2 (IL-2) generation and function were studied employing mononuclear cells from synovial fluid (SF), synovial tissue (ST) and peripheral blood (PB) of patients with rheumatoid arthritis (RA). Decreased PHA stimulated IL-2 production by lymphocytes from rheumatoid ST, SF (P < 0.02), and PB (P < 0.01) was observed when compared to normal blood and SF of patients with gout. The proliferative response of rheumatoid lymphocyte blasts exposed to exogenous IL-2 was also defective (P < 0.05-0.001). This defect was greater in SF than in rheumatoid PB (P < 0.05-0.001). In addition to the proliferative response, the effect of IL-2 on interferon-gamma (IFN-γ) production was also examined. Rheumatoid lymphocytes from both PB and SF produced less IFN-γ after overnight treatment with IL-2 than did normal PB lymphocytes. This decreased IFN-γ induction was discordant with the excellent enhancement by IL-2 of natural killer activity. Removal of adherent cells in synovial fluid did not correct this deficit. Abnormalities in the biology of IL-2 and IFN-γ suggest that impaired T cell function could contribute to the immunopathogenesis of RA.

Original languageEnglish (US)
Pages (from-to)520-528
Number of pages9
JournalClinical and Experimental Immunology
Volume59
Issue number3
StatePublished - Apr 24 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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