Interleukin-4 modulates collagen synthesis by human mesangial cells in a type-specific manner

Yuichi Nakazato*, Hirokazu Okada, Shogo Tajima, Tomoko Hayashida, Yoshihiko Kanno, Hiromichi Suzuki, Takao Saruta

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Several studies have suggested an in-volvement of interleukin (ID-4 in the pathophysiology of glomerulonephritis (GN). To elucidate its possible role in GN, we have investigated the effect of IL-4 on collagen accumulation by human mesangial cells (MC). After incubation with IL-la and/or IL-4 for 48 h, types I, III, and IV collagens in both soluble and cell-associated forms were identified by Western blotting. IL-lα stimulated type I and IV collagen synthesis, lacking significant effect on type collagen synthesis. In contrast, IL-4 stimulated type collagen synthesis without affecting type I and type IV synthesis. Enzyme-linked immunosorbent assay confirmed the dosedependent effect of IL-4 on collagen type secretion (2.0-fold at 10 ng/ml). Importantly, IL-1α-stimulated type I and IV collagen synthesis was suppressed by concomitant IL-4 treatment. Northern analysis of type I and procollagen mRNAs displayed consistent results. These data indicate that IL-4 selectively stimulates type collagen synthesis but also suppresses IL-1α-stimulated type I and IV collagen synthesis. Therefore IL-4 could potentially contribute to the pathological changes in glomerular diseases in cooperate with other cytokines.

Original languageEnglish (US)
Pages (from-to)F447-F453
JournalAmerican Journal of Physiology
Volume270
Issue number3 PART 2
DOIs
StatePublished - Jan 1 1996

Keywords

  • Glomerulonephritis
  • Interleukin-1
  • Type collagen
  • Western blot

ASJC Scopus subject areas

  • Physiology (medical)

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