Intermediate filament aggregates cause mitochondrial dysmotility and increase energy demands in giant axonal neuropathy

Eitan Israeli, Dilyan I. Dryanovski, Paul T. Schumacker, Navdeep S. Chandel, Jeffrey D. Singer, Jean P. Julien, Robert D. Goldman, Puneet Opal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Intermediate filaments (IFs) are cytoskeletal polymers that extend from the nucleus to the cell membrane, giving cells their shape and form. Abnormal accumulation of IFs is involved in the pathogenesis of number neurodegenerative diseases, but none as clearly as giant axonal neuropathy (GAN), a ravaging disease caused by mutations in GAN, encoding gigaxonin. Patients display early and severe degeneration of the peripheral nervous system along with IF accumulation, but it has been difficult to link GAN mutations to any particular dysfunction, in part because GAN null mice have a very mild phenotype. We therefore established a robust dorsal root ganglion neuronal model that mirrors key cellular events underlying GAN. We demonstrate that gigaxonin is crucial for ubiquitin-proteasomal degradation of neuronal IF. Moreover, IF accumulation impairs mitochondrial motility and is associated with metabolic and oxidative stress. These results have implications for other neurological disorders whose pathology includes IF accumulation.

Original languageEnglish (US)
Pages (from-to)2143-2157
Number of pages15
JournalHuman molecular genetics
Volume25
Issue number11
DOIs
StatePublished - Jun 1 2016

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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