TY - JOUR
T1 - Intermediate filament mechanics in vitro and in the cell
T2 - From coiled coils to filaments, fibers and networks
AU - Köster, Sarah
AU - Weitz, David A.
AU - Goldman, Robert D.
AU - Aebi, Ueli
AU - Herrmann, Harald
N1 - Funding Information:
S.K. thanks the German Research Foundation (DFG, KO 3752/5-1 and SFB 755 B07 and C10 ). H.H. received support from the German Research Foundation (DFG, HE 1853 , FOR1228 and 1853/11-1 ) and from COST. R.D.G. was supported by NIH PO1GM096971 and Hannah's Hope Fund. D.A.W. acknowledges support from the NIH ( PO1GM096971 ), the Harvard Materials Research Science and Engineering Center ( DMR-0820484 ), and the NSF ( DMR-1310266 ).
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Intermediate filament proteins form filaments, fibers and networks both in the cytoplasm and the nucleus of metazoan cells. Their general structural building plan accommodates highly varying amino acid sequences to yield extended dimeric α-helical coiled coils of highly conserved design. These 'rod' particles are the basic building blocks of intrinsically flexible, filamentous structures that are able to resist high mechanical stresses, that is, bending and stretching to a considerable degree, both in vitro and in the cell. Biophysical and computer modeling studies are beginning to unfold detailed structural and mechanical insights into these major supramolecular assemblies of cell architecture, not only in the 'test tube' but also in the cellular and tissue context.
AB - Intermediate filament proteins form filaments, fibers and networks both in the cytoplasm and the nucleus of metazoan cells. Their general structural building plan accommodates highly varying amino acid sequences to yield extended dimeric α-helical coiled coils of highly conserved design. These 'rod' particles are the basic building blocks of intrinsically flexible, filamentous structures that are able to resist high mechanical stresses, that is, bending and stretching to a considerable degree, both in vitro and in the cell. Biophysical and computer modeling studies are beginning to unfold detailed structural and mechanical insights into these major supramolecular assemblies of cell architecture, not only in the 'test tube' but also in the cellular and tissue context.
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U2 - 10.1016/j.ceb.2015.01.001
DO - 10.1016/j.ceb.2015.01.001
M3 - Review article
C2 - 25621895
AN - SCOPUS:84925060211
SN - 0955-0674
VL - 32
SP - 82
EP - 91
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
ER -