Interplay of cell-autonomous and nonautonomous mechanisms tailors synaptic connectivity of converging axons in vivo

Haruhisa Okawa, Luca Della Santina, Gregory W. Schwartz, Fred Rieke, Rachel O L Wong*

*Corresponding author for this work

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Neurons receive input from diverse afferents but form stereotypic connections with each axon type to execute their precise functions. Developmental mechanisms that specify the connectivity of individual axons across populations of converging afferents are not well-understood. Here, we untangled the contributions of activity-dependent and independent interactions that regulate the connectivity of afferents providing major and minor input onto a neuron. Individual transmission-deficient retinal bipolar cells (BCs) reduced synapses with retinal ganglion cells (RGCs), but active BCs of the same type sharing the dendrite surprisingly did not compensate for this loss. Genetic ablation of some BC neighbors resulted in increased synaptogenesis by the remaining axons in a transmission-independent manner. Presence, but not transmission, of the major BC input also dissuades wiring with the minor input and with synaptically compatible but functionally mismatched afferents. Cell-autonomous, activity-dependent and nonautonomous, activity-independent mechanisms thus together tailor connectivity of individual axons among converging inner retinal afferents.

Original languageEnglish (US)
Pages (from-to)125-137
Number of pages13
JournalNeuron
Volume82
Issue number1
DOIs
Publication statusPublished - Apr 2 2014

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ASJC Scopus subject areas

  • Neuroscience(all)

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