TY - JOUR
T1 - Interrogating genomic and epigenomic data to understand prostate cancer
AU - Kim, Jung
AU - Yu, Jindan
N1 - Funding Information:
We thank Dr. Hongjian Jin for helpful discussion. This work was supported in part by the NIH P50CA090386 Career Development Award (to J.Y.), U54CA143869 pilot project (to J.Y.), R00CA129565 (to J.Y.), and the U.S. Department of Defense PC080665 grant (to J.Y.), and the Research Scholar Grant # RSG-12-085-01 from the American Cancer Society (to J.Y.).
PY - 2012/4
Y1 - 2012/4
N2 - Major breakthroughs at the beginning of this century in high-throughput technologies have profoundly transformed biological research. Significant knowledge has been gained regarding our biological system and its disease such as malignant transformation. In this review, we summarize leading discoveries in prostate cancer research derived from the use of high-throughput approaches powered by microarrays and massively parallel next-generation sequencing (NGS). These include the seminal discovery of chromosomal translocations such as TMPRSS2-ERG gene fusions as well as the identification of critical oncogenes exemplified by the polycomb group protein EZH2. We then demonstrate the power of interrogating genomic and epigenomic data in understanding the plethora of mechanisms of transcriptional regulation. As an example, we review how androgen receptor (AR) binding events are mediated at multiple levels through protein-DNA interaction, histone and DNA modifications, as well as high-order chromatin structural changes.
AB - Major breakthroughs at the beginning of this century in high-throughput technologies have profoundly transformed biological research. Significant knowledge has been gained regarding our biological system and its disease such as malignant transformation. In this review, we summarize leading discoveries in prostate cancer research derived from the use of high-throughput approaches powered by microarrays and massively parallel next-generation sequencing (NGS). These include the seminal discovery of chromosomal translocations such as TMPRSS2-ERG gene fusions as well as the identification of critical oncogenes exemplified by the polycomb group protein EZH2. We then demonstrate the power of interrogating genomic and epigenomic data in understanding the plethora of mechanisms of transcriptional regulation. As an example, we review how androgen receptor (AR) binding events are mediated at multiple levels through protein-DNA interaction, histone and DNA modifications, as well as high-order chromatin structural changes.
KW - Androgen receptor
KW - EZH2
KW - FoxA1
KW - Gene fusion
KW - Integrative genomics
KW - Nucleosome positioning
KW - Transcriptional regulation
UR - http://www.scopus.com/inward/record.url?scp=84862783103&partnerID=8YFLogxK
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U2 - 10.1016/j.bbcan.2011.12.003
DO - 10.1016/j.bbcan.2011.12.003
M3 - Review article
C2 - 22240201
AN - SCOPUS:84862783103
SN - 0304-419X
VL - 1825
SP - 186
EP - 196
JO - Biochimica et Biophysica Acta - Reviews on Cancer
JF - Biochimica et Biophysica Acta - Reviews on Cancer
IS - 2
ER -
