Intersectional mapping of multi-transmitter neurons and other cell types in the brain

Jian Xu; Andrew Jo; Raina P. DeVries; Sercan Deniz; Suraj Cherian; Idris Sunmola; Xingqi Song; John J. Marshall; Katherine A. Gruner; Tanya L. Daigle; Anis Contractor; Talia N. Lerner; Hongkui Zeng; Yongling Zhu

doi:10.1016/j.celrep.2022.111036

Intersectional mapping of multi-transmitter neurons and other cell types in the brain

Jian Xu, Andrew Jo, Raina P. DeVries, Sercan Deniz, Suraj Cherian, Idris Sunmola, Xingqi Song, John J. Marshall, Katherine A. Gruner, Tanya L. Daigle, Anis Contractor, Talia N. Lerner, Hongkui Zeng, Yongling Zhu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Recent developments in intersectional strategies have greatly advanced our ability to precisely target brain cell types based on unique co-expression patterns. To accelerate the application of intersectional genetics, we perform a brain-wide characterization of 13 Flp and tTA mouse driver lines and selected seven for further analysis based on expression of vesicular neurotransmitter transporters. Using selective Cre driver lines, we created more than 10 Cre/tTA combinational lines for cell type targeting and circuit analysis. We then used VGLUT-Cre/VGAT-Flp combinational lines to identify and map 30 brain regions containing neurons that co-express vesicular glutamate and gamma-aminobutyric acid (GABA) transporters, followed by tracing their projections with intersectional viral vectors. Focusing on the lateral habenula (LHb) as a target, we identified glutamatergic, GABAergic, or co-glutamatergic/GABAergic innervations from ∼40 brain regions. These data provide an important resource for the future application of intersectional strategies and expand our understanding of the neuronal subtypes in the brain.

Original language	English (US)
Article number	111036
Journal	Cell reports
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/j.celrep.2022.111036
State	Published - Jul 5 2022

Funding

We thank Steven H. DeVries for discussions and critical reading and editing the manuscript. This work was supported by NIH grants R01 EY030169 (Y.Z.), R01 EY018204 (Y.Z.), R01 MH099114 (A.C.), T32 EY025202 (A.J.), F31 EY031985 (A.J.), Whitehall Foundation Grant (Y.Z., 2017-05-20 ), and Research to Prevent Blindness .

Keywords

CP: Neuroscience
brain mapping
intersectional strategy
multi-transmitter neurons
the lateral habenula

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2022.111036

Cite this

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title = "Intersectional mapping of multi-transmitter neurons and other cell types in the brain",

abstract = "Recent developments in intersectional strategies have greatly advanced our ability to precisely target brain cell types based on unique co-expression patterns. To accelerate the application of intersectional genetics, we perform a brain-wide characterization of 13 Flp and tTA mouse driver lines and selected seven for further analysis based on expression of vesicular neurotransmitter transporters. Using selective Cre driver lines, we created more than 10 Cre/tTA combinational lines for cell type targeting and circuit analysis. We then used VGLUT-Cre/VGAT-Flp combinational lines to identify and map 30 brain regions containing neurons that co-express vesicular glutamate and gamma-aminobutyric acid (GABA) transporters, followed by tracing their projections with intersectional viral vectors. Focusing on the lateral habenula (LHb) as a target, we identified glutamatergic, GABAergic, or co-glutamatergic/GABAergic innervations from ∼40 brain regions. These data provide an important resource for the future application of intersectional strategies and expand our understanding of the neuronal subtypes in the brain.",

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AU - DeVries, Raina P.

AU - Deniz, Sercan

AU - Cherian, Suraj

AU - Sunmola, Idris

AU - Song, Xingqi

AU - Marshall, John J.

AU - Gruner, Katherine A.

AU - Daigle, Tanya L.

AU - Contractor, Anis

AU - Lerner, Talia N.

AU - Zeng, Hongkui

AU - Zhu, Yongling

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Intersectional mapping of multi-transmitter neurons and other cell types in the brain

Abstract

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Keywords

ASJC Scopus subject areas

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