Intracellular delivery of HSP70 using HIV-1 Tat protein transduction domain

Derek S. Wheeler, Katherine E. Dunsmore, Hector R. Wong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Heat shock protein 70 (HSP70) is an intracellular stress protein that confers cytoprotection to a variety of cellular stressors. Several lines of evidence have suggested that augmentation of the heat shock response by increasing the expression of HSP70 represents a potential therapeutic strategy for the treatment of critically ill patients. The Tat protein of human immunodeficiency virus 1 (HIV-1) has been used previously to deliver functional cargo proteins intracellularly when added exogenously to cultured cells. We generated a Tat-HSP70 fusion protein using recombinant methods and treated HSF -/- cells with either Tat-HSP70 or recombinant HSP70 prior to exposure to hyperoxia or lethal heat shock. We showed that biologically active, exogenous HSP70 can be delivered into cells using the HIV-1 Tat protein, and that the Tat-mediated delivery of HSP70 confers cytoprotection against thermal stress and hyperoxia and may represent a novel approach to augmenting intracellular HSP70 levels.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume301
Issue number1
DOIs
StatePublished - Jan 31 2003
Externally publishedYes

Keywords

  • Heat shock factor
  • Heat shock protein (hsp)-70
  • Heat shock response
  • Hyperoxia
  • Protein transduction
  • Tat

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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