Intracellular distribution of TiO2-DNA oligonucleotide nanoconjugates directed to nucleolus and mitochondria indicates sequence specificity

Tatjana Paunesku; Stefan Vogt; Barry Lai; Jörg Maser; Nataša Stojićević; Kenneth T. Thurn; Clodia Osipo; Hong Liu; Daniel Legnini; Zhou Wang; Chung Lee; Gayle E. Woloschak

doi:10.1021/nl0624723

Intracellular distribution of TiO₂-DNA oligonucleotide nanoconjugates directed to nucleolus and mitochondria indicates sequence specificity

Tatjana Paunesku, Stefan Vogt, Barry Lai, Jörg Maser, Nataša Stojićević, Kenneth T. Thurn, Clodia Osipo, Hong Liu, Daniel Legnini, Zhou Wang, Chung Lee, Gayle E. Woloschak^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

Deoxyribonucleic acid (DNA) oligonucleotides hybridize to matching DNA sequences in cells, as established in the literature, depending on active transcription of the target sequence and local molarity of the oligonucleotide. We investigated the intracellular distribution of nanoconjugates composed of DNA oligonucleotides attached to TiO₂ nanoparticles, thus creating a locally increased concentration of the oligonucleotide. Two types of nanoconjugates, with oligonucleotides matching mitochondrial or nucleolar DNA, were specifically retained in mitochondria or nucleoli.

Original language	English (US)
Pages (from-to)	596-601
Number of pages	6
Journal	Nano letters
Volume	7
Issue number	3
DOIs	https://doi.org/10.1021/nl0624723
State	Published - Mar 2007

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl0624723

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title = "Intracellular distribution of TiO2-DNA oligonucleotide nanoconjugates directed to nucleolus and mitochondria indicates sequence specificity",

abstract = "Deoxyribonucleic acid (DNA) oligonucleotides hybridize to matching DNA sequences in cells, as established in the literature, depending on active transcription of the target sequence and local molarity of the oligonucleotide. We investigated the intracellular distribution of nanoconjugates composed of DNA oligonucleotides attached to TiO2 nanoparticles, thus creating a locally increased concentration of the oligonucleotide. Two types of nanoconjugates, with oligonucleotides matching mitochondrial or nucleolar DNA, were specifically retained in mitochondria or nucleoli.",

author = "Tatjana Paunesku and Stefan Vogt and Barry Lai and J{\"o}rg Maser and Nata{\v s}a Stoji{\'c}evi{\'c} and Thurn, {Kenneth T.} and Clodia Osipo and Hong Liu and Daniel Legnini and Zhou Wang and Chung Lee and Woloschak, {Gayle E.}",

year = "2007",

month = mar,

doi = "10.1021/nl0624723",

language = "English (US)",

volume = "7",

pages = "596--601",

journal = "Nano Letters",

issn = "1530-6984",

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T1 - Intracellular distribution of TiO2-DNA oligonucleotide nanoconjugates directed to nucleolus and mitochondria indicates sequence specificity

AU - Paunesku, Tatjana

AU - Vogt, Stefan

AU - Lai, Barry

AU - Maser, Jörg

AU - Stojićević, Nataša

AU - Thurn, Kenneth T.

AU - Osipo, Clodia

AU - Liu, Hong

AU - Legnini, Daniel

AU - Wang, Zhou

AU - Lee, Chung

AU - Woloschak, Gayle E.

PY - 2007/3

Y1 - 2007/3

N2 - Deoxyribonucleic acid (DNA) oligonucleotides hybridize to matching DNA sequences in cells, as established in the literature, depending on active transcription of the target sequence and local molarity of the oligonucleotide. We investigated the intracellular distribution of nanoconjugates composed of DNA oligonucleotides attached to TiO2 nanoparticles, thus creating a locally increased concentration of the oligonucleotide. Two types of nanoconjugates, with oligonucleotides matching mitochondrial or nucleolar DNA, were specifically retained in mitochondria or nucleoli.

AB - Deoxyribonucleic acid (DNA) oligonucleotides hybridize to matching DNA sequences in cells, as established in the literature, depending on active transcription of the target sequence and local molarity of the oligonucleotide. We investigated the intracellular distribution of nanoconjugates composed of DNA oligonucleotides attached to TiO2 nanoparticles, thus creating a locally increased concentration of the oligonucleotide. Two types of nanoconjugates, with oligonucleotides matching mitochondrial or nucleolar DNA, were specifically retained in mitochondria or nucleoli.

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JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 3

ER -

Intracellular distribution of TiO₂-DNA oligonucleotide nanoconjugates directed to nucleolus and mitochondria indicates sequence specificity

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