Intracellular transport and storage of secretory proteins in relation to cytodifferentiation in neoplastic pancreatic acinar cells

M. J. Becich, M. Bendayan, J. K. Reddy

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The pancreatic acinar carcinoma established in rat by Reddy and Rao demonstrates heterogeneity of cytodifferentiation ranging from cells containing abundant well-developed secretory granules to those with virtually none. We examined the synthesis of intracellular transport and storage of secretory proteins in secretory granule-enriched (GEF) and secretory granule-deficient (GDF) subpopulations of neoplastic acinar cells separable by Percoll gradient centrifugation, to determine the secretory process in cells with distinctly different cytodifferentiation. The cells pulse-labeled with [3H]leucine for 3 min and chase incubated for up to 4 h were analyzed by quantitative electron microscope autoradiography. In GEF neoplastic cells, the results of grain counts and relative grain density estimates establish that the label moves successively from rough endoplasmic reticulum (RER) → the Golgi apparatus → post-Golgi vesicles (vacuoles or immature vacuoles) → mature secretory granules, in a manner reminiscent of the secretory process in normal pancreatic acinar cells. The presence of ~40% of the label in association with secretory granules at 4 h postpulse indicates that GEF neoplastic cells retain (acquire) the essential regulatory controls of the secretory process. In GDF neoplastic acinar cells the drainage of label from RER is slower, but the peak label of ~20% in the Golgi apparatus is reached relatively rapidly (10 min postpulse). The movement of label from the Golgi to the post-Golgi vesicles is evident; further delineation of the secretory process in GDF neoplastic cells, however, was not possible due to lack of secretory granule differentiation. The movement of label from RER → the Golgi apparatus → the post-Golgi vesicles suggests that GDF neoplastic cells also synthesize secretory proteins, but to a lesser extent than the GEF cells. The reason(s) for the inability of GDF cells to concentrate and store exportable proteins remain to be elucidated.

Original languageEnglish (US)
Pages (from-to)949-960
Number of pages12
JournalJournal of Cell Biology
Volume96
Issue number4
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Cell Biology

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