Intracellular Uncaging of cGMP with Blue Light

Hitesh K. Agarwal, Shenyu Zhai, D. James Surmeier, Graham C.R. Ellis-Davies*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We have made a new caged cGMP that is photolyzed with blue light. Using our recently developed derivative of 7-diethylaminocourmarin (DEAC) called DEAC450, we synthesized coumarin phosphoester derivatives of cGMP with two negative charges appended to the DEAC450 moiety. DEAC450-cGMP is freely soluble in physiological buffer without the need for any organic cosolvents. With a photolysis quantum yield of 0.18 and an extinction coefficient of 43 000 M-1 cm-1 at 453 nm, DEAC450-cGMP is the most photosensitive caged cGMP made to date. In patch-clamped neurons in acutely isolated brain slices, blue light effectively uncaged cGMP from DEAC450 and facilitated activation of hyperpolarization and cyclic nucleotide gated cation (HCN) channels in cholinergic interneurons. Thus, DEAC450-cGMP has a unique set of optical and chemical properties that make it a useful addition to the optical arsenal available to neurobiologists.

Original languageEnglish (US)
Pages (from-to)2139-2144
Number of pages6
JournalACS Chemical Neuroscience
Volume8
Issue number10
DOIs
StatePublished - Oct 18 2017

Keywords

  • Caged compounds
  • HCN channels
  • blue light
  • cGMP
  • coumarin
  • neurophysiology

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cognitive Neuroscience
  • Cell Biology

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