Intranasal delivery of therapeutic stem cells to glioblastoma in a mouse model

Dou Yu, Gina Li, Maciej S. Lesniak, Irina V. Balyasnikova*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The intrinsic tropism towards brain malignancies renders stem cells as promising carriers of therapeutic agents against malignant tumors. The delivery of therapeutic stem cells via the intranasal route is a recently discovered alternative strategy, with strong potential for clinical translation, due to its non-invasive nature compared to intracranial implantation or delivery via systemic routes. The lack of blood brain barrier further strengthens the therapeutic potential of stem cells undergoing intranasal brain entry. This article summarizes the essential techniques utilized in our studies and outlines the basic principles of intranasal strategy for stem cell delivery using a mouse model of intracranial glioma xenografts. We demonstrate the optimized procedures that generate consistent and reproducible results with specific predetermined experimental parameters and offer guidelines for streamlined work flow that ensure efficient execution and reliable experimental outcome. The article is designed to serve as a baseline for further experimental customization based on hypothesis, stem cell types, or tumor specifics.

Original languageEnglish (US)
Article numbere55845
JournalJournal of Visualized Experiments
Volume2017
Issue number124
DOIs
StatePublished - Jun 4 2017

Keywords

  • Brain tumor
  • Genetic modification
  • Hypoxia
  • Intranasal delivery
  • Issue 124
  • Medicine
  • Oncolytic virus
  • Stem cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Intranasal delivery of therapeutic stem cells to glioblastoma in a mouse model'. Together they form a unique fingerprint.

Cite this