Intrapleural low-molecular-weight urokinase or tissue plasminogen activator versus single-chain urokinase in tetracycline-induced pleural loculation in rabbits

Steven Idell*, Ali Azghani, Shande Chen, Kathy Koenig, Andrew Mazar, Lalitha Kodandapani, Khalil Bdeir, Douglas Cines, Irina Kulikovskaya, Timothy Allen

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The authors compared the ability of a single dose of the proenzyme single-chain urokinase (scuPA), low-molecular-weight urokinase, tissue plasminogen activator (tPA), or a mutant site-inactive scuPA to resolve intrapleural loculations at 72 to 96 hours after tetracycline-induced pleural injury in rabbits. Both scuPA and tPA reversed loculations at 96 hours after injury P ≤ .001, whereas low-molecular-weight urokinase and the scuPA mutant were ineffective. scuPA and tPA generated inhibitor complexes, induced fibrinolytic activity, and quenched plasminogen activator-1 activity in pleural fluids. The authors conclude that scuPA reverses loculations as effectively as tPA at clinically applied intrapleural doses, whereas low-molecular-weight urokinase was ineffective.

Original languageEnglish (US)
Pages (from-to)419-440
Number of pages22
JournalExperimental Lung Research
Volume33
Issue number8-9
DOIs
StatePublished - Oct 1 2007

Keywords

  • Fibrinolysis
  • Loculation
  • Pleurodesis
  • Tissue plasminogen activator
  • Urokinase

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

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    Idell, S., Azghani, A., Chen, S., Koenig, K., Mazar, A., Kodandapani, L., Bdeir, K., Cines, D., Kulikovskaya, I., & Allen, T. (2007). Intrapleural low-molecular-weight urokinase or tissue plasminogen activator versus single-chain urokinase in tetracycline-induced pleural loculation in rabbits. Experimental Lung Research, 33(8-9), 419-440. https://doi.org/10.1080/01902140701703333