Intrinsic properties and mechanisms of spontaneous firing in mouse cerebellar unipolar brush cells

Marco J. Russo, Enrico Mugnaini, Marco Martina*

*Corresponding author for this work

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Neuronal firing patterns are determined by the cell's intrinsic electrical and morphological properties and are regulated by synaptic interactions. While the properties of cerebellar neurons have generally been studied in much detail, little is known about the unipolar brush cells (UBCs), a type of glutamatergic interneuron that is enriched in the granular layer of the mammalian vestibulocerebellum and participates in the representation of head orientation in space. Here we show that UBCs can be distinguished from adjacent granule cells on the basis of differences in membrane capacitance, input resistance and response to hyperpolarizing current injection. We also show that UBCs are intrinsically firing neurons. Using action potential clamp experiments and whole-cell recordings we demonstrate that two currents contribute to this property: a persistent TTX-sensitive sodium current and a ruthenium red-sensitive, TRP-like cationic current, both of which are active during interspike intervals and have reversal potentials positive to threshold. Interestingly, although UBCs are also endowed with a large Ih current, this current is not involved in their intrinsic firing, perhaps because it activates at voltages that are more hyperpolarized than those associated with autonomous activity.

Original languageEnglish (US)
Pages (from-to)709-724
Number of pages16
JournalJournal of Physiology
Volume581
Issue number2
DOIs
StatePublished - Jun 1 2007

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Neurons
Ruthenium Red
Patch-Clamp Techniques
Interneurons
Action Potentials
Sodium
Head
Injections
Membranes

ASJC Scopus subject areas

  • Physiology

Cite this

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Intrinsic properties and mechanisms of spontaneous firing in mouse cerebellar unipolar brush cells. / Russo, Marco J.; Mugnaini, Enrico; Martina, Marco.

In: Journal of Physiology, Vol. 581, No. 2, 01.06.2007, p. 709-724.

Research output: Contribution to journalArticle

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