Intrinsic spectrally-dependent background in spectroscopic visible-light optical coherence tomography

Ian Rubinoff, Roman V. Kuranov, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Visible-light optical coherence tomography (vis-OCT) has enabled new spectroscopic applications, such as retinal oximetry, as a result of increased optical absorption and scattering contacts in biological tissue and improved axial resolution. Besides extracting tissue properties from back-scattered light, spectroscopic analyses must consider spectral alterations induced by image reconstruction itself. We investigated an intrinsic spectral bias in the background noise floor, which is hereby referred to as the spectrally-dependent background (SDBG). We developed an analytical model to predict the SDBG-induced bias and validated this model using numerically simulated and experimentally acquired data. We found that SDBG systemically altered the measured spectra of blood in human retinal vessels in vis-OCT, as compared to literature data. We provided solutions to quantify and compensate for SDBG in retinal oximetry. This work is particularly significant for clinical applications of vis-OCT.

Original languageEnglish (US)
Pages (from-to)110-124
Number of pages15
JournalBiomedical Optics Express
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2021

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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