Introduction to RNA-seq and its applications to drug discovery and development

Zainab Khatoon, Bryan Figler, Hui Zhang, Feng Cheng*

*Corresponding author for this work

Research output: Contribution to journalReview article

21 Scopus citations

Abstract

RNA sequencing (RNA-Seq) is a novel high-throughput technology for comprehensive transcriptome study. It can measure the expression levels of thousands of genes simultaneously and provide insight into functional pathways and regulations in biological processes. In addition, RNA-Seq can provide copious information on alternative splicing, allele-specific expression, unannotated exons, and novel transcripts (gene or noncoding RNAs). This technology has revolutionized the way biologists examine transcriptomes and has been successfully applied in drug discovery and development, being able to identify drug-related genes, microRNAs, and fusion proteins. In this overview, we will review this technology including data analysis, and its recent applications in drug discovery and development.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalDrug Development Research
Volume75
Issue number5
DOIs
StatePublished - Aug 2014

Keywords

  • RNA-Seq
  • drug resistance
  • fusion protein
  • miRNA
  • microarray
  • next-generation sequencing

ASJC Scopus subject areas

  • Drug Discovery

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